{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:40:42Z","timestamp":1788871242249,"version":"build-2803163510"},"reference-count":34,"publisher":"Georg Thieme Verlag KG","issue":"08","funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["HL49141, HL76457, HL04239"],"award-info":[{"award-number":["HL49141, HL76457, HL04239"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2005]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Human blood platelets have important, regulatory functions in diverse hemostatic and pathological disorders, including vascular remodeling, inflammation, and wound repair. Microarray analysis was used to study the molecular basis of essential thrombocythemia, a myeloproliferative disorder with quantitative and qualitative platelet defects associated with cardiovascular and thrombohemorrhagic symptoms, not infrequently neurological. A platelet-expressed gene (HSD17B3) encoding type 3 17\u03b2-hydroxysteroid dehydrogenase (previously characterized as a testis-specific enzyme catalyzing the final step in gonadal synthesis of testosterone) was selectively down-regulated in ET platelets, with reciprocal induction of the type 12 enzyme (HSD17B12). Functional 17\u03b2-HSD3 activity corresponding to \u223c10% of that found in murine testis was demonstrated in normal platelets. The induction of HSD17B12 in ET platelets was unassociated with a concomitant increase in androgen biosynthesis, suggesting distinct functions and\/or substrate specificities of the types 3 and 12 enzymes. Application of a molecular assay distinguished ET from normal platelets in 20 consecutive patients (p &lt; 0.0001). These data provide the first evidence that distinct subtypes of steroidogenic 17\u03b2\u2212HSDs are functionally present in human blood platelets, and that the expression patterns of HSD17B3 and HSD17B12 are associated with an uncommon platelet disorder manifest by quantitative and qualitative platelet defects.<\/jats:p>","DOI":"10.1160\/th05-01-0037","type":"journal-article","created":{"date-parts":[[2005,7,14]],"date-time":"2005-07-14T09:52:10Z","timestamp":1121334730000},"page":"412-421","source":"Crossref","is-referenced-by-count":58,"title":["Platelets express steroidogenic 17\u03b2\u2013hydroxysteroid dehydrogenases"],"prefix":"10.1160","volume":"94","author":[{"given":"Dmitri V.","family":"Gnatenko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa D.","family":"Cupit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily C.","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anilkumar","family":"Dhundale","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. Perrotta","family":"Peter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wadie F.","family":"Bahou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2017,12,5]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.1182\/blood-2002-09-2797","article-title":"Transcript profiling of human platelets using microarray and serial analysis of gene expression","volume":"101","author":"DV Gnatenko","year":"2003","journal-title":"Blood"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1182\/blood.V93.2.415","article-title":"Essential thrombocythemia: Another \u2018\u2018heterogeneous disease\u201d better understood?","volume":"93","author":"SD Nimer","year":"1999","journal-title":"Blood"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"128.","DOI":"10.1182\/blood.V89.1.128","article-title":"Clonality analysis of hematopoiesis in essential thrombocythemia: Advantages of studying T lymphocytes and platelets","volume":"89","author":"N El-Kassar","year":"1997","journal-title":"Blood"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1091.","DOI":"10.1182\/blood.V92.4.1091","article-title":"Familial essential thrombocythemia associated with one-base deletion in the 5\u2019-untranslated region of the thrombopoietin gene","volume":"92","author":"T Kondo","year":"1998","journal-title":"Blood"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1056\/NEJMra035363","article-title":"Thrombocytosis","volume":"350","author":"AI Schafer","year":"2004","journal-title":"N Engl J Med"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1055\/s-2004-833482","article-title":"Platelet transcriptome: the application of microarray analysis to platelets","volume":"30","author":"WF Bahou","year":"2004","journal-title":"Semin Thromb Hemost"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/ajh.2830250209","article-title":"Differentiation between essential thrombocythemia and polycythemia vera with marked thrombocytosis","volume":"25","author":"HJ Iland","year":"1987","journal-title":"Am J Hematol"},{"key":"ref8","first-page":"29","article-title":"Experience of the Polycythemia Vera Study Group with essential thrombocythemia: a final report on diagnostic criteria, survival, and leukemic transition by treatment","volume":"34","author":"S Murphy","year":"1997","journal-title":"Semin Hematol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1172\/JCI118597","article-title":"The proteinase activated receptor-2 (PAR-2) mediates mitogenic responses in human vascular endothelial cells. 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