{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:35:55Z","timestamp":1740137755306,"version":"3.37.3"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"07","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Sciences Foundation of PR China","doi-asserted-by":"crossref","award":["10402002 and 30670904"],"award-info":[{"award-number":["10402002 and 30670904"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003181","name":"Foundation for the Author of National Excellent Doctoral Dissertation of PR China","doi-asserted-by":"crossref","award":["200356"],"award-info":[{"award-number":["200356"]}],"id":[{"id":"10.13039\/501100003181","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2008]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The binding of plasma von Willebrand factor (vWF) to platelet glycoprotein (GP) Ib\u03b1 in a high shear stress field, and subsequent integrin-GPIIb\/IIIa-vWF conjunction induces platelet aggregation (SIPA). However, the specific biomechanical mechanism of the vWF-GPIb interaction still remains to be elucidated. A parallel-plate rectangular flow chamber was built to simulate a stenopeic artery flow pattern. Using the flow chamber, we examined shear- induced platelet activation (SIPAct) at different vWF concentrations (5\u201325 \u00b5g\/ml) and several simulated stenotic high shear rates. P-selectin expression on the platelets and annexin V binding to the platelets were used as two markers of platelet activation. At different localized shear rates (3,000 s-1\u20139,500 s-1), the percentage of annexin V and P-selectin positive cells increased from 8.3 \u00b1 0.4% to 22.3 \u00b1 1.8% ( p 0.05) and from 17.4 \u00b1 0.5% to 33.5 \u00b1 2.5% (p 0.05),respectively. As the vWF concentration increased from 5 \u00b5g\/ml to 25 \u00b5g\/ml, the annexinV binding rate increased from 7.2 \u00b1 0.6% to 53.4 \u00b1 3.8% (p 0.05), and P-selectin expression increased from 16.5 \u00b1 1.2% to 65.9 \u00b1 5.2% (p 0.05). A test in a uniform shear field using cone-plate viscometer rheometry showed that the platelet activation rate was proportional to the platelet concentration. This result suggests that platelet collision is one of the impact factors of SIPAct.<\/jats:p>","DOI":"10.1160\/th07-03-0222","type":"journal-article","created":{"date-parts":[[2008,6,11]],"date-time":"2008-06-11T05:50:42Z","timestamp":1213163442000},"page":"60-68","source":"Crossref","is-referenced-by-count":6,"title":["Effects of von Willebrand factor concentration and platelet collision on shear-induced platelet activation"],"prefix":"10.1160","volume":"100","author":[{"given":"Zhenyue","family":"Gao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziqiang","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Bai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengyuan","family":"Zhuang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changgeng","family":"Ruan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2017,11,22]]},"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1160\/TH07-03-0222.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,3,22]],"date-time":"2018-03-22T06:44:02Z","timestamp":1521701042000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1160\/TH07-03-0222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008]]},"references-count":0,"journal-issue":{"issue":"07","published-online":{"date-parts":[[2017,11,22]]},"published-print":{"date-parts":[[2008]]}},"URL":"https:\/\/doi.org\/10.1160\/th07-03-0222","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"type":"print","value":"0340-6245"},{"type":"electronic","value":"2567-689X"}],"subject":[],"published":{"date-parts":[[2008]]}}}