{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,5,3]],"date-time":"2022-05-03T09:05:54Z","timestamp":1651568754959},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2010]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In critically ill patients, the massive release of angiopoietin-2 (Ang-2) from Weibel-Palade bodies interferes with protective angiopoietin-1 (Ang-1)\/Tie2 signalling in endothelial cells, thus leading to vascular inflammation and subsequent organ-dysfunction. We hypothesised that plasma exchange (PE) is efficient for lowering excess Ang-2 levels in critically ill patients with thrombocytic microangiopathy (TMA) or anti-glomerular basement membrane (anti-GBM) disease. Plasma Ang-1 and Ang-2 were measured by immuno-luminometric assays in patients with TMA (n=9) or anti-GBM disease (n=4) before and after up to four PE sessions. Twenty apparently healthy volunteers served as controls. Median (IQR) plasma levels of Ang-2 were markedly increased in patients with TMA (7.3 (2.4\u201321.1) ng\/ml) and anti-GBM disease (5.8 (3.4\u20137.0) ng\/ml) compared to healthy controls (1.0 (0.9\u20131.4) ng\/ml, p &lt;0.001). Moreover, Ang-1 plasma levels were decreased in both, TMA (1.02 (0.62\u20131.62) ng\/ml) and anti-GBM disease patients (0.74 (0.59\u20133.62) ng\/ml) compared to healthy controls (2.5 (1.93\u20133.47) ng\/ ml, p &lt;0.005). During a total of 32 treatments, PE effectively lowered elevated mean (SD) Ang-2 plasma levels by 36.7 \u00b1 19.6 % per treatment (p &lt;0.0001), whereas low Ang-1 plasma levels remained unchanged (0.3 \u00b1 58.5 %; p =0.147). Ang-2 levels declined to almost normal values during \u22644 PE treatments (Friedman\u00b4s test p &lt;0.0001). PE is an effective method to remove excess circulating Ang-2. It remains to be elucidated if the removal of Ang-2 is crucial to ameliorate endothelial damage in critically ill patients with severely altered endothelial integrity.<\/jats:p>","DOI":"10.1160\/th10-02-0138","type":"journal-article","created":{"date-parts":[[2010,8,31]],"date-time":"2010-08-31T04:38:38Z","timestamp":1283229518000},"page":"1038-1043","source":"Crossref","is-referenced-by-count":10,"title":["Removal of elevated circulating angiopoietin-2 by plasma exchange \u2013 A pilot study in critically ill patients with thrombotic microangiopathy and anti-glomerular basement membrane disease"],"prefix":"10.1160","volume":"104","author":[{"given":"Carsten","family":"Hafer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Kielstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Haubitz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"Haller","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svjetlana","family":"Lovric","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Lukasz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp","family":"K\u00fcmpers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2017,11,24]]},"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1160\/TH10-02-0138.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,3,22]],"date-time":"2018-03-22T10:04:24Z","timestamp":1521713064000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1160\/TH10-02-0138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010]]},"references-count":0,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,11,24]]},"published-print":{"date-parts":[[2010]]}},"URL":"https:\/\/doi.org\/10.1160\/th10-02-0138","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010]]}}}