{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:21:04Z","timestamp":1760955664050},"reference-count":20,"publisher":"Georg Thieme Verlag KG","issue":"01","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Patients with type 3 von Willebrand disease (VWD-3) have no measurable levels of VW factor (VWF) and usually require treatment with VWF-FVIII concentrate to prevent and\/or stop bleeding. Even though the patients are treated prophylactically, they may experience bleeding symptoms. The aim of this study was to evaluate the effect of VWF-FVIII concentrate treatment in VWD-3 patients with the Total Thrombus Analysis System (T-TAS\u00ae), which measures thrombus formation under flow conditions. Coagulation profiles of 10 VWD-3 patients were analysed using T-TAS before and 30 minutes after VWF-FVIII concentrate (Haemate\u00ae) injection. Results were compared to VWF- and FVIII activity in plasma, and results with thromboelastometry and ris-tocetin-activated platelet impedance aggregometry (Multiplate\u00ae) in whole blood. For comparison, 10 healthy controls were also analysed with T-TAS. A median dose of 27 (range 15\u201335) IU\/kg of VWF-FVIII concentrate increased VWF- and FVIII activity as expected. T-TAS thrombus formation was enhanced when a tissue factor\/collagen-coated flow chamber was used at low shear, but treatment effects at high shear using a collagen-coated flow chamber were minimal. Whole blood coagulation assessed by thromboelastometry was normal and did not change (p &gt; 0.05) but ristocetin-induced platelet aggregation improved (p &lt; 0.001). In conclusion, T-TAS detects effects of VWF-FVIII concentrate treatment on coagulation-dependent thrombus formation at low shear, but minor effects are observed on platelet-dependent thrombus formation at high shear. The poor prediction of bleeding by conventional laboratory monitoring in VWD-3 patients might be related to insufficient restoration of platelet-dependent thrombus formation.<\/jats:p>","DOI":"10.1160\/th16-06-0430","type":"journal-article","created":{"date-parts":[[2016,10,20]],"date-time":"2016-10-20T07:12:50Z","timestamp":1476947570000},"page":"75-85","source":"Crossref","is-referenced-by-count":11,"title":["Monitoring of coagulation factor therapy in patients with von Willebrand disease type 3 using a microchip flow chamber system"],"prefix":"10.1160","volume":"117","author":[{"given":"Margareta","family":"Holmstr\u00f6m","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David E.","family":"Schmidt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuya","family":"Hosokawa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margareta","family":"Blomb\u00e4ck","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Hjemdahl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"\u00c5gren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2017,11,10]]},"reference":[{"key":"10.1160\/TH16-06-0430-1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1383546"},{"key":"10.1160\/TH16-06-0430-2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13243"},{"key":"10.1160\/TH16-06-0430-3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.02146.x"},{"key":"10.1160\/TH16-06-0430-4","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1281033"},{"key":"10.1160\/TH16-06-0430-5","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-08-528398"},{"key":"10.1160\/TH16-06-0430-6","doi-asserted-by":"publisher","DOI":"10.1160\/TH13-11-0952"},{"key":"10.1160\/TH16-06-0430-7","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13273"},{"key":"10.1160\/TH16-06-0430-8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04464.x"},{"key":"10.1160\/TH16-06-0430-9","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23599"},{"key":"10.1160\/TH16-06-0430-10","doi-asserted-by":"publisher","DOI":"10.1160\/TH12-06-0441"},{"key":"10.1160\/TH16-06-0430-11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2010.03975.x"},{"key":"10.1160\/TH16-06-0430-12","doi-asserted-by":"publisher","DOI":"10.1111\/hae.12610"},{"key":"10.1160\/TH16-06-0430-13","doi-asserted-by":"publisher","DOI":"10.1007\/s002770050542"},{"key":"10.1160\/TH16-06-0430-14","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112064"},{"key":"10.1160\/TH16-06-0430-15","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2014.03.024"},{"key":"10.1160\/TH16-06-0430-16","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1182\/blood.V77.9.1901.1901","volume":"77","author":"Castillo","year":"1991","journal-title":"Blood"},{"key":"10.1160\/TH16-06-0430-17","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-949667"},{"key":"10.1160\/TH16-06-0430-18","first-page":"CD010438","volume":"2","author":"Hunt","year":"2015","journal-title":"Cochrane Database Syst Rev"},{"key":"10.1160\/TH16-06-0430-19","doi-asserted-by":"publisher","DOI":"10.1532\/IJH97.06214"},{"key":"10.1160\/TH16-06-0430-20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04400.x"}],"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1160\/TH16-06-0430.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T20:13:57Z","timestamp":1601151237000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1160\/TH16-06-0430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":20,"journal-issue":{"issue":"01","published-online":{"date-parts":[[2017,11,14]]},"published-print":{"date-parts":[[2017]]}},"URL":"https:\/\/doi.org\/10.1160\/th16-06-0430","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}