{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:22:12Z","timestamp":1773300132383,"version":"3.50.1"},"reference-count":54,"publisher":"Georg Thieme Verlag KG","issue":"09","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Platelet degranulation at the site of vascular injury prevents bleeding and may affect the chronic vascular wound healing response. Transforming Growth Factor (TGF)-\u03b21 is a major component of platelet \u03b1-granules known to accumulating in thrombi. It was our aim to determine the role of TGF\u03b21 released from activated platelets for neointima formation following arterial injury and thrombosis. Mice with platelet-specific deletion of TGF\u03b21 (Plt.TGF\u03b2-KO) underwent carotid artery injury. Immunoassays confirmed the absence of active TGF\u03b21 in platelet releasates and plasma of Plt.TGF\u03b2-KO mice. Whole blood analyses revealed similar haematological parameters, and tail cut assays excluded major bleeding defects. Platelet aggregation and the acute thrombotic response to injury in vivo did not differ between Plt.TGF\u03b2-KO and Plt.TGF\u03b2-WT mice. Morphometric analysis revealed that absence of TGF\u03b21 in platelets resulted in a significant reduction of neointima formation with lower neointima area, intima-to-media ratio, and lumen stenosis. On the other hand, the media area was enlarged in mice lacking TGF\u03b21 in platelets and contained increased amounts of proteases involved in latent TGF\u03b2 activation, including MMP2, MMP9 and thrombin. Significantly increased numbers of proliferating cells and cells expressing the mesenchymal markers platelet-derived growth factor receptor-\u03b2 or fibroblast-specific protein-1, and the macrophage antigen F4\/80, were observed in the media of Plt.TGF\u03b2-KO mice, whereas the medial smooth muscle-actin-immuno-positive area and collagen content did not differ between genotypes. Our findings support an essential role for platelet-derived TGF\u03b21 for the vascular remodelling response to arterial injury, apparently independent from the role of platelets in thrombosis or haemostasis.<\/jats:p><jats:p>Supplementary Material to this article is available online at www.thrombosis-online.com.<\/jats:p>","DOI":"10.1160\/th17-02-0112","type":"journal-article","created":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T13:24:53Z","timestamp":1500557093000},"page":"1782-1797","source":"Crossref","is-referenced-by-count":10,"title":["Absence of transforming growth factor beta 1 in murine platelets reduces neointima formation without affecting arterial thrombosis"],"prefix":"10.1160","volume":"117","author":[{"given":"Eva","family":"Sch\u00fctz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena 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