{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T18:15:21Z","timestamp":1790100921746,"version":"4.0.1"},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>\n            Background\u2003Replacement therapy with coagulation factor VIII (FVIII) concurrent with bleeds (on-demand) in haemophilia A (HA) patients has been hypothesized to increase the risk for antidrug antibodies (inhibitors). A danger signal environment, characterized by tissue damage and inflammation at the site of a bleed, is thought to contribute to the anti-FVIII response. The nature of this inflammatory reaction is, however, not fully known, and new insights will be valuable for both managing inhibitors and understanding arthropathy development.<\/jats:p><jats:p>\n            Objective\u2003To characterize the inflammatory response, locally and systemically, during the first 24 hours following a joint bleed in the HA rat.<\/jats:p><jats:p>\n            Methods\u2003HA rats received a needle-induced knee joint bleed (n\u2009=\u200983) or a sham procedure (n\u2009=\u200941). Blood samples were collected at selected time points from 0 to 24 hours post injury\/sham. Synovial fluid, intra-articular knee tissue and popliteal lymph nodes were collected at 24 hours. Cytokine\/chemokine concentrations and gene expression were measured.<\/jats:p><jats:p>\n            Results\u2003Gene expression analysis revealed a rapid inflammatory response in the injured knees, accompanied by significantly increased levels of specific gene products in the synovial fluid; IL-1\u03b2, TNF\u03b1, KC\/GRO, IL-6, Eotaxin, MCP-1, MCP-3, MIP-1\u03b1, MIP-2, RANTES, A2M and AGP. Plasma analysis demonstrated significantly increased systemic levels of KC\/GRO and IL-6 in injured rats already after 5 to 6 hours.<\/jats:p><jats:p>\n            Conclusion\u2003A rapid proinflammatory response, locally and systemically, characteristic of innate immunity, was demonstrated. Results reveal a more comprehensive inflammatory picture than previously shown, with resemblance to human haemophilic arthropathy, and with unique correlation between gene expression level, synovial concentration and plasma concentration in individual rats.<\/jats:p>","DOI":"10.1160\/th17-03-0149","type":"journal-article","created":{"date-parts":[[2017,12,20]],"date-time":"2017-12-20T01:56:29Z","timestamp":1513734989000},"page":"2092-2104","source":"Crossref","is-referenced-by-count":10,"title":["Acute Haemarthrosis in the Haemophilia A Rat Generates a Local and Systemic Proinflammatory Response"],"prefix":"10.1160","volume":"117","author":[{"given":"Karin","family":"L\u00f6vgren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristine","family":"Christensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiktor","family":"Majewski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"\u00d8strup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren","family":"Skov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Wiinberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2017,11,30]]},"container-title":["Thrombosis and Haemostasis"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.1160\/TH17-03-0149.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T15:38:53Z","timestamp":1557157133000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.1160\/TH17-03-0149"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":0,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2017,11,30]]},"published-print":{"date-parts":[[2017,11]]}},"URL":"https:\/\/doi.org\/10.1160\/th17-03-0149","relation":{},"ISSN":["0340-6245","2567-689X"],"issn-type":[{"value":"0340-6245","type":"print"},{"value":"2567-689X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}