{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T20:22:26Z","timestamp":1767903746721,"version":"3.49.0"},"reference-count":47,"publisher":"Georg Thieme Verlag KG","issue":"10","funder":[{"name":"Spanish Ministry of Economy and Competitiveness of Science","award":["SAF2016\u201376819-R"],"award-info":[{"award-number":["SAF2016\u201376819-R"]}]},{"name":"Institute of Health Carlos III, ISCIII","award":["TERCEL RD16\/00110018 and CB16\/11\/0041 to L. B.; and FIS PI16\/01915 to T. P."],"award-info":[{"award-number":["TERCEL RD16\/00110018 and CB16\/11\/0041 to L. B.; and FIS PI16\/01915 to T. P."]}]},{"DOI":"10.13039\/501100002809","name":"Government of Catalonia","doi-asserted-by":"crossref","award":["2014SGR1303 to L. B."],"award-info":[{"award-number":["2014SGR1303 to L. B."]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Thromb Haemost"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The role of erythrocytes in thrombus formation has been often neglected, but some studies have highlighted their active role in thrombotic events. Free-haemoglobin (Hb) has shown to induce oxidative-stress damage. Herein we have investigated the coordinated changes in heme-related proteins in patients with acute-coronary-syndromes (ACS), their association to ongoing thrombosis and their impact on patients\u2019 prognosis. The serum proteome of STEMI-patients (N=27) within the first 6h after event-onset and 3d after were compared to controls (N=60). Changes in heme-metabolism were characterized in a second STEMI-group by a dual proteomic approach analyzing in-vivo aspirated coronary thrombi at PCI (N=24) and the associated peripheral-blood changes (N=10). A third STEMI-group (N=132) was studied to analyze the impact of the observed changes in prognosis at 6-months-follow-up. The haptoglobin\/hemopexin(Hpg\/Hpx)-scavenging-system revealed a time-dependent response after STEMI with an early increase in Hpg circulating levels in the acute phase (P=0.01) and a late increase in Hpx levels 3d after (P=0.045). Beta-Hb content in coronary thrombi was directly correlated with systemic beta-Hb and Hpg (R=0.804,P=0.0029; R=0.859,P=0.0007) levels. The presence of a fully-occlusive thrombus was associated to higher circulating levels of beta-Hb (P=0.03) and unbound-Hpg (P=0.03). ELISA validation demonstrated a decreased survival rate at 6-months follow-up in STEMI-patients with lower Hpg plasma levels at admission (P=0.027). Our results show active Hb-release form erythrocytes in ACS. This release is followed by a systemic early increase in Hpg levels and a late increase in Hpx levels that can co-ordinately help to prevent systemic pro-oxidative effects. The Hb-scavenging ability of haptoglobin is related to patients\u2019 prognosis.<\/jats:p><jats:p>Supplementary Material to this article is available online at www.thrombosis-online.com.<\/jats:p>","DOI":"10.1160\/th17-05-0314","type":"journal-article","created":{"date-parts":[[2017,7,27]],"date-time":"2017-07-27T05:56:07Z","timestamp":1501134967000},"page":"1970-1980","source":"Crossref","is-referenced-by-count":11,"title":["Erythrocyte-heme proteins and STEMI: implications in 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