{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:53:04Z","timestamp":1787017984087,"version":"build-2736575974"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arteriosclerosis"],"published-print":{"date-parts":[[1990,5]]},"abstract":"<jats:p>We studied the effects of calcium, cyclic nucleotides, and protein kinase C on albumin transfer, electrical resistance, and cytoskeletal actin filaments in cultured human umbilical vein endothelial cells. The endothelial monolayer grown on collagen-treated filters markedly restricted the transfer of albumin relative to its transfer across the filter alone. Both Ca++ ionophore A23187 and ethyleneglycol tetraacetic acid disrupted the integrity of the endothelial monolayer, thereby increasing endothelial albumin transfer and decreasing electrical resistance in a concentration-dependent manner. Neither W-7, a calmodulin antagonist, nor TMB-8, an intracellular Ca++ antagonist, influenced endothelial permeability. In contrast, increases in intracellular cyclic adenosine 5'-monophosphate (AMP) and\/or cyclic guanosine 5'-monophosphate (GMP) induced by dibutyryl cyclic AMP, forskolin, 3-isobutyl-1-methylxanthine, 8-bromo cyclic GMP, dibutyryl cyclic GMP, or sodium nitroprusside significantly elevated endothelial electrical resistance and inhibited albumin transfer; similar effects resulted from activation of protein kinase C by phorbol-12-myristate-13-acetate or 1-oleoyl-2-acetyl-glycerol. These substances ruffled the dense peripheral bands of F-actin without compromising the integrity of endothelial monolayer. These results suggest that calcium, cyclic nucleotides, and protein kinase C play important roles in the regulation of endothelial permeability and the maintenance of endothelial integrity.<\/jats:p>","DOI":"10.1161\/01.atv.10.3.410","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T15:44:19Z","timestamp":1308325459000},"page":"410-420","source":"Crossref","is-referenced-by-count":48,"title":["Roles of calcium, cyclic nucleotides, and protein kinase C in regulation of endothelial permeability."],"prefix":"10.1161","volume":"10","author":[{"given":"Y","family":"Yamada","sequence":"first","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Furumichi","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Furui","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Yokoi","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Ito","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Yamauchi","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Yokota","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Hayashi","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Saito","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Nagoya University School of Medicine, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Arteriosclerosis: An Official Journal of the American Heart Association, Inc."],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.ATV.10.3.410","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T10:18:00Z","timestamp":1647512280000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.10.3.410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,5]]}},"alternative-id":["10.1161\/01.ATV.10.3.410"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.10.3.410","relation":{},"ISSN":["0276-5047"],"issn-type":[{"value":"0276-5047","type":"print"}],"subject":[],"published":{"date-parts":[[1990,5]]}}}