{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T20:39:39Z","timestamp":1782160779048,"version":"3.54.5"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arterioscler Thromb"],"published-print":{"date-parts":[[1993,11]]},"abstract":"<jats:p>During development of atherosclerotic lesions, vascular smooth muscle cells (SMCs) undergo changes both phenotypically and in their cytoskeleton composition. An expression of cytokeratins 8 and 18 in SMCs in plaques of the human superficial femoral artery and of cytokeratin 8 in lesions of the aorta was recently described. Since cytokeratins are epithelial markers generally not found in normal adult vascular SMCs, we performed a detailed immunofluorescence microscopy study using a large panel of antibodies against the various cytokeratin polypeptides and other elements of the cytoskeleton. We included lesions of carotid, common and superficial femoral, iliac, and popliteal arteries; the abdominal aorta; and saphenous vein bypass grafts, as well as primary, restenotic, and transplantation-associated lesions of coronary arteries (n = 33). Cytokeratins 8 and 18 were present in myointimal cells of all pathological specimens. Colocalization with smooth muscle alpha-actin identified most cytokeratin-positive cells as SMCs. Only very few cells cosynthesized cytokeratin and desmin, whereas the majority of cytokeratin-positive cells were vimentin-positive. This pattern of cytoskeletal protein synthesis is similar to that found in some fetal and\/or neonatal SMCs. These findings suggest that the synthesis of cytokeratins in a subset of SMCs of atherosclerotic lesions is a common phenomenon in coronary artery and peripheral vascular disease as well as graft disease and transplantation-associated arteriosclerosis and that the state of these SMCs is of a \"dedifferentiated\" fetal type.<\/jats:p>","DOI":"10.1161\/01.atv.13.11.1631","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"1631-1639","source":"Crossref","is-referenced-by-count":38,"title":["Cytokeratins 8 and 18 in smooth muscle cells. Detection in human coronary artery, peripheral vascular, and vein graft disease and in transplantation-associated arteriosclerosis."],"prefix":"10.1161","volume":"13","author":[{"given":"L","family":"Jahn","sequence":"first","affiliation":[{"name":"Molecular Medicine and Cardiovascular Division, Beth Israel Hospital, Boston Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Kreuzer","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Cardiovascular Division, Beth Israel Hospital, Boston Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"von Hodenberg","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Cardiovascular Division, Beth Israel Hospital, Boston Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"K\u00fcbler","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Cardiovascular Division, Beth Israel Hospital, Boston Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W W","family":"Franke","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Cardiovascular Division, Beth Israel Hospital, Boston Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Allenberg","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Cardiovascular Division, Beth Israel Hospital, Boston Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Izumo","sequence":"additional","affiliation":[{"name":"Molecular Medicine and Cardiovascular Division, Beth Israel Hospital, Boston Mass."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Arteriosclerosis and Thrombosis: A Journal of Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.ATV.13.11.1631","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T14:21:24Z","timestamp":1647526884000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.13.11.1631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1993,11]]}},"alternative-id":["10.1161\/01.ATV.13.11.1631"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.13.11.1631","relation":{},"ISSN":["1049-8834"],"issn-type":[{"value":"1049-8834","type":"print"}],"subject":[],"published":{"date-parts":[[1993,11]]}}}