{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:34:01Z","timestamp":1773290041359,"version":"3.50.1"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arterioscler Thromb"],"published-print":{"date-parts":[[1993,11]]},"abstract":"<jats:p>Tissue factor (TF) is the predominant physiological initiator of coagulation, and its regulation is a critical aspect of endothelial cell hemostatic function. This report describes the regulation of TF mRNA expression by two physiological agonists: minimally oxidized low-density lipoprotein (MM-LDL), which may modulate endothelial hemostatic function in atherosclerosis, and lipopolysaccharide (LPS), which is a mediator of septic shock. Northern blot analysis of total RNA from human endothelial cells exposed to either MM-LDL or LPS for varying times showed that TF mRNA increased sharply at 1 hour, peaked at 2 to 3 hours, and declined to basal levels by 6 to 8 hours after treatment. The half-life of TF mRNA in MM-LDL- and LPS-exposed endothelial cells was approximately 45 minutes and 40 minutes, respectively. The rate of TF mRNA degradation was similar at 1 and 4 hours after exposure in either MM-LDL- or LPS-stimulated endothelial cells. Nuclear runoff transcription assays showed a significantly increased rate of TF gene transcription in both MM-LDL- and LPS-exposed endothelial cells. Cycloheximide inhibited the induction of TF protein activity, but it enhanced the accumulation of TF mRNA in MM-LDL- and LPS-induced endothelial cells. These results indicated that regulation of TF expression by MM-LDL and LPS in human endothelial cells occurs principally at the level of gene transcription.<\/jats:p>","DOI":"10.1161\/01.atv.13.11.1711","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"1711-1717","source":"Crossref","is-referenced-by-count":49,"title":["Regulation of endothelial cell tissue factor expression by minimally oxidized LDL and lipopolysaccharide."],"prefix":"10.1161","volume":"13","author":[{"given":"H","family":"Fei","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, UCLA School of Medicine 90024-1732."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Berliner","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, UCLA School of Medicine 90024-1732."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Parhami","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, UCLA School of Medicine 90024-1732."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T A","family":"Drake","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, UCLA School of Medicine 90024-1732."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Arteriosclerosis and Thrombosis: A Journal of Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.ATV.13.11.1711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T14:21:42Z","timestamp":1647526902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.13.11.1711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1993,11]]}},"alternative-id":["10.1161\/01.ATV.13.11.1711"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.13.11.1711","relation":{},"ISSN":["1049-8834"],"issn-type":[{"value":"1049-8834","type":"print"}],"subject":[],"published":{"date-parts":[[1993,11]]}}}