{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T14:19:36Z","timestamp":1648649976314},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arterioscler Thromb"],"published-print":{"date-parts":[[1993,3]]},"abstract":"<jats:p>We have observed that NPC-15669, a leucine derivative with anti-inflammatory activity, reduced the proliferation of human aortic smooth muscle cells (HASMCs) in culture. We used a colorimetric assay and tritiated thymidine to measure the cell density and proliferation of HASMC cultures treated with this agent. We also studied the effect of NPC-15669 on the proliferation and migration of human aortic endothelial cells (HAECs). Subconfluent HASMC cultures were growth arrested for 2 days. On the third day, growth was stimulated with either growth media (medium M199 containing 10% fetal bovine serum [FBS]), human platelet-derived growth factor (hPDGF), or fibroblast growth factor (FGF) in the absence or presence of NPC-15669 (0.1-50 microM). Regardless of the stimulating agent for HASMCs (FBS, hPDGF, or FGF), NPC-15669 at concentrations of 10-25 microM caused a significant reduction in thymidine incorporation (36.7% and 77.2% in 10 microM and 25 microM, respectively; p &lt; 0.005) and cell density (25-87%, p &lt; 0.001) compared with control. NPC-15669 did not, however, have an effect on the rate of proliferation or migration of HAECs, even at concentrations up to 50 microM. Two other anti-inflammatory agents, aspirin and dexamethasone, caused substantially and significantly less inhibition, even at high concentrations (50 and 25 microM, respectively). This study demonstrates that in vitro, NPC-15669 significantly inhibits HASMC proliferation but has no effect on proliferation or migration of HAECs.<\/jats:p>","DOI":"10.1161\/01.atv.13.3.360","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"360-366","source":"Crossref","is-referenced-by-count":6,"title":["A new anti-inflammatory leucine derivative, NPC-15669, inhibits growth of cultured human aortic smooth muscle cells."],"prefix":"10.1161","volume":"13","author":[{"given":"R L","family":"Bennett","sequence":"first","affiliation":[{"name":"Division of Cardiology, UCLA School of Medicine 90024-1679."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Navab","sequence":"additional","affiliation":[{"name":"Division of Cardiology, UCLA School of Medicine 90024-1679."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L L","family":"Demer","sequence":"additional","affiliation":[{"name":"Division of Cardiology, UCLA School of Medicine 90024-1679."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A M","family":"Fogelman","sequence":"additional","affiliation":[{"name":"Division of Cardiology, UCLA School of Medicine 90024-1679."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Arteriosclerosis and Thrombosis: A Journal of Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.ATV.13.3.360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T17:37:06Z","timestamp":1647538626000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.13.3.360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,3]]}},"alternative-id":["10.1161\/01.ATV.13.3.360"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.13.3.360","relation":{},"ISSN":["1049-8834"],"issn-type":[{"value":"1049-8834","type":"print"}],"subject":[],"published":{"date-parts":[[1993,3]]}}}