{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:48:38Z","timestamp":1758278918644},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arterioscler Thromb"],"published-print":{"date-parts":[[1993,8]]},"abstract":"<jats:p>This report describes the normalized intimal-medial uptakes [uptake (M, mg.cm-2) divided serum concentration (c0, mg.cm-3)] of 125I-albumin, 125I-low-density lipoprotein (LDL), and in vivo Evans blue dye (EBD)-albumin complex as functions of pressure (P), time (t), molecular species (i), and location (z) along a ventral longitudinal z axis of the normal, intact, aortic endothelial surface in adult normocholesterolemic Sinclair Research Farm (SRF) minipigs and compares these uptake (M\/c0) measurements with atherogenesis in hypercholesterolemic cohorts. Uptakes of porcine serum 125I-albumin (n = 21) and 125I-LDL (n = 10) were measured in freshly excised, metabolically supported aortas using a recently developed organ-support system. In vivo intimal-medial EBD uptake vs z data were measured photometrically on opened descending aortas from another group (n = 6) of normocholesterolemic, adult, SRF minipigs 18 hours after the intravenous administration of EBD. For comparison purposes, the corresponding incidence of atherosclerotic lesions along the aortic z axis was calculated using topographic data from hypercholesterolemic minipig cohorts (n = 39). The results showed that uptakes varied greatly with t, z, and macromolecule (i) but not with P. More specifically, the value of M\/c0 at any location (z) rose with t, was insensitive to P, decreased with macromolecular (i) size, and varied with z in a pattern that \"peaked\" in the upstream region, decreased to a nadir in the downstream region, and then rose again as it approached the abdominal celiac orifice. The spatially z-averaged uptake rates for the three different labeled serum proteins were 0.31 x 10(-3) cm.h-1 for 125I-albumin, 0.42 x 10(-3) cm.h-1 for EBD-albumin, and 0.04 x 10(-3) cm.h-1 for 125I-LDL. Nondimensionalized analysis of the individual sets of uptake data indicated that the overall uptake relationship [M(t,P,z,i)\/c(io), cm] could be characterized empirically by the simple product of two separate functions: one, a \"scaling function\" [m(z,i)], that described the uptake magnitude for a given i and z and appeared to be independent of t or P; the other, a \"shape function\" [s(t,P)], that described the shapes of the uptake vs t and P relationships and appeared to be independent of z or i. The \"scaling function\" [m(z,i)] vs z contour appeared to correlate well with the corresponding atherosclerotic lesion incidence vs z contour from the group of hypercholesterolemic minipig cohorts. Assuming passive transport, it was shown (\"Appendix\") that m(z,i) can be interpreted physically in terms of an endothelial diffusive permeability coefficient (P,cm.s-1).(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1161\/01.atv.13.8.1193","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"1193-1204","source":"Crossref","is-referenced-by-count":19,"title":["Local intimal-medial uptakes of 125I-albumin, 125I-LDL, and parenteral Evans blue dye protein complex along the aortas of normocholesterolemic minipigs as predictors of subsequent hypercholesterolemic atherogenesis."],"prefix":"10.1161","volume":"13","author":[{"given":"D L","family":"Fry","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Atherosclerosis, College of Medicine, Ohio State University, Columbus 43210-1214."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E E","family":"Herderick","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Atherosclerosis, College of Medicine, Ohio State University, Columbus 43210-1214."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D K","family":"Johnson","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Atherosclerosis, College of Medicine, Ohio State University, Columbus 43210-1214."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Arteriosclerosis and Thrombosis: A Journal of Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.ATV.13.8.1193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T13:51:39Z","timestamp":1647525099000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.13.8.1193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,8]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1993,8]]}},"alternative-id":["10.1161\/01.ATV.13.8.1193"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.13.8.1193","relation":{},"ISSN":["1049-8834"],"issn-type":[{"value":"1049-8834","type":"print"}],"subject":[],"published":{"date-parts":[[1993,8]]}}}