{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:37:35Z","timestamp":1790847455080,"version":"4.1.0"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[1995,8]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            The promoter region of the endothelial cell nitric oxide synthase (ecNOS) gene contains potential response elements for transforming growth factor\u2013\u03b2\n            <jats:sub>1<\/jats:sub>\n            (TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            ). TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            plays an important role in the pathogenesis of atherosclerosis, vascular hypertrophy, and angiogenesis. We therefore sought to determine whether TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            might modulate ecNOS expression in bovine aortic endothelial cells (BAEC). TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            increased ecNOS mRNA in a dose-dependent manner. TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            also increased ecNOS protein content. The production of nitrogen oxides (NO\n            <jats:sub>x<\/jats:sub>\n            ), assessed by chemiluminescence, and nitric oxide synthase activity, assessed by arginine\/citrulline conversion, were increased in TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            -treated cells. Transcriptional activity of the 5\u2032-flanking promoter region of the ecNOS gene was increased by TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            , as assessed by transfection with promoter\/luciferase constructs. Deletion analysis suggested that the TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            -response element was present between nucleotides \u22121269 and \u2212935 from the first transcription start site, in which a putative nuclear factor\u20131 (NF-1) binding site existed. Gel shift assays showed that nuclear protein(s), immunologically similar to CCAAT transcription factor\/NF-1, bound to the putative NF-1 binding site in a sequence-specific manner. Mutation of the putative NF-1 binding site in the promoter\/luciferase construct significantly decreased the responsiveness to TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            . In conclusion, TGF\u03b2\n            <jats:sub>1<\/jats:sub>\n            increases ecNOS expression associated with an increase in production of NO in BAEC. This response is probably mediated by transcriptional activation of the ecNOS gene promoter.\n          <\/jats:p>","DOI":"10.1161\/01.atv.15.8.1255","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"1255-1261","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":142,"title":["Molecular Regulation of the Bovine Endothelial Cell Nitric Oxide Synthase by Transforming Growth Factor\u2013\u03b2\n            <sub>1<\/sub>"],"prefix":"10.1161","volume":"15","author":[{"given":"Nobutaka","family":"Inoue","sequence":"first","affiliation":[{"name":"From the Departments of Medicine and Pharmacology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Ga."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard C.","family":"Venema","sequence":"additional","affiliation":[{"name":"From the Departments of Medicine and Pharmacology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Ga."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan S.","family":"Sayegh","sequence":"additional","affiliation":[{"name":"From the Departments of Medicine and Pharmacology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Ga."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Ohara","sequence":"additional","affiliation":[{"name":"From the Departments of Medicine and Pharmacology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Ga."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. J.","family":"Murphy","sequence":"additional","affiliation":[{"name":"From the Departments of Medicine and Pharmacology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Ga."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Harrison","sequence":"additional","affiliation":[{"name":"From the Departments of Medicine and Pharmacology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Ga."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/327524a0"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/345161a0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116092"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49528-8"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42066-2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.14.6348"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)85359-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4781(94)90195-3"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1994.1068"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.73.1.7685252"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.5.C1381"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/362801a0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116027"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114029"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.12.4167"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(88)80033-3"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.12.4.1846"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.4.1492"},{"key":"e_1_3_2_19_2","first-page":"H1030","volume":"256","year":"1990","journal-title":"Am J Physiol"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.83.4.1707354"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.5.1475"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90358-2"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2740799"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90583-6"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90358-8"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/334218a0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"13625","DOI":"10.1016\/S0021-9258(19)38694-6","volume":"268","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.81.6.2111743"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3461562"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91209-X"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114949"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3175638"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.11.4651"},{"key":"e_1_3_2_34_2","first-page":"H862","volume":"265","year":"1993","journal-title":"Am J Physiol"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2164258"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.ATV.15.8.1255","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T17:28:05Z","timestamp":1647538085000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.15.8.1255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8]]},"references-count":35,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1995,8]]}},"alternative-id":["10.1161\/01.ATV.15.8.1255"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.15.8.1255","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,8]]}}}