{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:33:09Z","timestamp":1791091989825,"version":"4.1.0"},"reference-count":61,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:p><jats:italic>Abstract<\/jats:italic>The amino acid sequence of interferon gamma (IFN-\u03b3) has basic amino acid clusters similar to the heparin-binding consensus sequences found in other proteins that bind to proteoglycans (PGs). We investigated whether recombinant human IFN-\u03b3 could bind to extracellular matrix (ECM) PGs secreted by human arterial smooth muscle cells (HASMCs) in vitro and whether the interaction affected the cellular response to IFN-\u03b3. As an in vitro model of ECM we used the basement membrane from HASMCs in culture. The binding of<jats:sup>125<\/jats:sup>I-IFN-\u03b3 to ECM was reduced significantly by pretreatment of ECM with chondroitinase ABC, an enzyme that degrades chondroitin-sulfate glycosaminoglycans. IFN-\u03b3 binding to ECM was reduced by increasing concentrations of chondroitin-6-sulfate.<jats:sup>125<\/jats:sup>I-IFN-\u03b3 (0.05 to 2 ng\/mL) binding data indicated an apparent<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>of 2\u00d710<jats:sup>\u221211<\/jats:sup>mol\/L and a maximum binding of 1.6\u00d710<jats:sup>6<\/jats:sup>IFN-\u03b3 molecules bound per square millimeter of ECM. Experiments with synthetic peptides suggested that residues 127 through 135 (AKTGKRKRS) are involved in the binding. The binding to chondroitin-sulfate PGs was confirmed by affinity chromatography of isolated [<jats:sup>35<\/jats:sup>S]chondroitin-sulfate PGs from ECM and cell-culture medium on immobilized IFN-\u03b3. The binding was abolished by treatment with chondroitinase ABC. ECM-bound IFN-\u03b3 was more effective in inducing the expression of class II major histocompatibility antigens such as HLA-DR in HASMCs and human arterial endothelial cells than soluble IFN-\u03b3. These results suggest a role for chondroitin-sulfate PGs in immobilizing IFN-\u03b3 in the ECM compartment and enhancing the cellular response to IFN-\u03b3.<\/jats:p>","DOI":"10.1161\/01.atv.15.9.1456","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"1456-1465","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":30,"title":["Interferon Gamma Binds to Extracellular Matrix Chondroitin-Sulfate Proteoglycans, Thus Enhancing Its Cellular Response"],"prefix":"10.1161","volume":"15","author":[{"given":"Eva Hurt","family":"Camejo","sequence":"first","affiliation":[{"name":"From the Wallenberg Laboratory for Cardiovascular Research, University of Gothenburg, Sahlgrenska University Hospital, and the Biochemistry Department Preclinical Research Laboratories (G.C.), Astra Ha\u0308ssle, Mo\u0308lndal, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitta","family":"Rosengren","sequence":"additional","affiliation":[{"name":"From the Wallenberg Laboratory for Cardiovascular Research, University of Gothenburg, Sahlgrenska University Hospital, and the Biochemistry Department Preclinical Research Laboratories (G.C.), Astra Ha\u0308ssle, Mo\u0308lndal, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Germa\u0301n","family":"Camejo","sequence":"additional","affiliation":[{"name":"From the Wallenberg Laboratory for Cardiovascular Research, University of Gothenburg, Sahlgrenska University Hospital, and the Biochemistry Department Preclinical Research Laboratories (G.C.), Astra Ha\u0308ssle, Mo\u0308lndal, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Sartipy","sequence":"additional","affiliation":[{"name":"From the Wallenberg Laboratory for Cardiovascular Research, University of Gothenburg, Sahlgrenska University Hospital, and the Biochemistry Department Preclinical Research Laboratories (G.C.), Astra Ha\u0308ssle, Mo\u0308lndal, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnar","family":"Fager","sequence":"additional","affiliation":[{"name":"From the Wallenberg Laboratory for Cardiovascular Research, University of Gothenburg, Sahlgrenska University Hospital, and the Biochemistry Department Preclinical Research Laboratories (G.C.), Astra Ha\u0308ssle, Mo\u0308lndal, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Go\u0308ran","family":"Bondjers","sequence":"additional","affiliation":[{"name":"From the Wallenberg Laboratory for Cardiovascular Research, University of Gothenburg, Sahlgrenska University Hospital, and the Biochemistry Department Preclinical Research Laboratories (G.C.), Astra Ha\u0308ssle, Mo\u0308lndal, Sweden."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(85)90080-5"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.11.040193.003035"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8456300"},{"key":"e_1_3_2_4_2","first-page":"243","volume":"69","year":"1990","journal-title":"Immunology"},{"key":"e_1_3_2_5_2","first-page":"169","volume":"135","year":"1989","journal-title":"Am J Pathol"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.63.4.3139321"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113998"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/atv91.11.5.1911708"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.23.10530"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(88)90324-3"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112475"},{"key":"e_1_3_2_12_2","first-page":"121","volume":"135","year":"1989","journal-title":"Am J Pathol"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1990.70.2.427"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.3.689"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(90)90024-8"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115548"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.1.217"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(92)90053-J"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/362801a0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.88.4.7691431"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/326403a0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00430a047"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"16812","DOI":"10.1016\/S0021-9258(18)41855-8","volume":"267","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830221139"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/332376a0"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02631088"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.116.2.533"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115093"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-199310000-00007"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90308-L"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","unstructured":"Wight TN Heinega\u030ard DK Hascall VC. Proteoglycans: structure and function. In: Hay ED ed. Cell Biology of Extracellular Matrix . 2nd ed. New York NY: Plenum Press; 1991:45-71.","DOI":"10.1007\/978-1-4615-3770-0_3"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.9.1.21"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1991.71.2.481"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1902591"},{"key":"e_1_3_2_35_2","first-page":"511","volume":"25","year":"1988","journal-title":"In Vitro Cell Dev Biol"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","unstructured":"Thyberg J Hedin U Bottger BA. Attachment substrate for smooth muscle cells. In: Piper HM ed. Cell Culture Techniques in Heart and Vessel Research . Berlin FRG: Springer; 1991:315-333.","DOI":"10.1007\/978-3-642-75262-9_21"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114272"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90586-9"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","unstructured":"Hulme EC Birdsall NJM. Strategy and tactics in receptor-binding studies. In: Receptor-Ligand Interactions: A Practical Approach . Hulme EC ed. New York NY: Oxford University Press; 1992.","DOI":"10.1093\/oso\/9780199630905.001.0001"},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"14131","DOI":"10.1016\/S0021-9258(19)85218-3","volume":"268","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_3_2_41_2","first-page":"167","volume":"96","year":"1993","journal-title":"J Cell Biol"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4800(85)90088-7"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(88)90032-9"},{"key":"e_1_3_2_44_2","first-page":"1858","volume":"7","year":"1993","journal-title":"Biochemistry"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.8.4.368"},{"key":"e_1_3_2_46_2","first-page":"1","volume":"9","year":"1988","journal-title":"Arteriosclerosis"},{"key":"e_1_3_2_47_2","first-page":"72","volume":"45","year":"1987","journal-title":"Eur J Cell Biol"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409239209082560"},{"key":"e_1_3_2_49_2","doi-asserted-by":"crossref","first-page":"20419","DOI":"10.1016\/S0021-9258(20)80745-5","volume":"268","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_3_2_50_2","unstructured":"Nathan C Yoshida R. Cytokines: interferon-\u03b3. In: Gallin JI Goldstein IM Snyderman R eds. Inflammation: Basic Principles and Clinical Correlates . New York NY: Raven Press Ltd; 1988:229-251."},{"key":"e_1_3_2_51_2","doi-asserted-by":"crossref","first-page":"19228","DOI":"10.1016\/S0021-9258(19)36503-2","volume":"268","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/4.3.335"},{"key":"e_1_3_2_53_2","doi-asserted-by":"crossref","first-page":"8534","DOI":"10.1016\/S0021-9258(19)83943-1","volume":"261","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1991.tb16481.x"},{"key":"e_1_3_2_55_2","doi-asserted-by":"crossref","first-page":"3360","DOI":"10.4049\/jimmunol.139.10.3360","volume":"139","year":"1987","journal-title":"J Immunol"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.55.11.2727-2733.1987"},{"key":"e_1_3_2_57_2","doi-asserted-by":"crossref","first-page":"13314","DOI":"10.1016\/S0021-9258(19)38300-0","volume":"265","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_3_2_58_2","doi-asserted-by":"crossref","first-page":"6023","DOI":"10.1016\/S0021-9258(18)38076-1","volume":"266","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.25.11831"},{"key":"e_1_3_2_60_2","first-page":"151","volume":"280","year":"1991","journal-title":"FEBS Lett"},{"key":"e_1_3_2_61_2","doi-asserted-by":"crossref","unstructured":"Ruoslahti E. Integrins as receptors for extracellular matrix. In: Hay ED ed. Cell Biology of Extracellular Matrix . New York NY: Plenum Press; 1991:343-359.","DOI":"10.1007\/978-1-4615-3770-0_11"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.ATV.15.9.1456","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,7]],"date-time":"2024-04-07T17:41:56Z","timestamp":1712511716000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.15.9.1456"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,9]]},"references-count":61,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1995,9]]}},"alternative-id":["10.1161\/01.ATV.15.9.1456"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.15.9.1456","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,9]]}}}