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Incubation of Hep G2 cells with gemfibrozil resulted in the following statistically significant findings: (1) increased accumulation of apoA-I in the medium without affecting uptake of radiolabeled HDL-protein or HDL\u2013apoA-I; (2) accelerated incorporation of [\n            <jats:sup>3<\/jats:sup>\n            H]leucine and [\n            <jats:sup>35<\/jats:sup>\n            S]methionine into apoA-I; (3) equivalent increases in [\n            <jats:sup>3<\/jats:sup>\n            H]leucine incorporation into HDL particles without and with apoA-II (LpA-I and LpA-I+A-II, respectively); (4) equal efflux of fibroblast cholesterol by harvested LpA-I and LpA-I+A-II particles; (5) increased steady state apoA-I mRNA without affecting apoA-I transcription; and (6) increased apoA-I mRNA half-life (2.2-fold). These data indicate that gemfibrozil stabilizes apoA-I mRNA transcripts, resulting in increased translation of functional apoA-I\u2013containing particles capable of effluxing cellular cholesterol, thus defining a major mechanism by which gemfibrozil increases HDL.\n          <\/jats:p>","DOI":"10.1161\/01.atv.16.8.1052","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"1052-1062","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":27,"title":["Gemfibrozil Stimulates Apolipoprotein A-I Synthesis and Secretion by Stabilization of mRNA Transcripts in Human Hepatoblastoma Cell Line (Hep G2)"],"prefix":"10.1161","volume":"16","author":[{"given":"Fu-You","family":"Jin","sequence":"first","affiliation":[{"name":"the Cholesterol Center, Medical Service, Long Beach (Calif) Department of Veterans Affairs Medical Center, and the University of California, Irvine."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vaijinath S.","family":"Kamanna","sequence":"additional","affiliation":[{"name":"the Cholesterol Center, Medical Service, Long Beach (Calif) Department of Veterans Affairs Medical Center, and the University of California, Irvine."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-Yu","family":"Chuang","sequence":"additional","affiliation":[{"name":"the Cholesterol Center, Medical Service, Long Beach (Calif) Department of Veterans Affairs Medical Center, and the University of California, Irvine."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kengathevy","family":"Morgan","sequence":"additional","affiliation":[{"name":"the Cholesterol Center, Medical Service, Long Beach (Calif) Department of Veterans Affairs Medical Center, and the University of California, Irvine."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moti L.","family":"Kashyap","sequence":"additional","affiliation":[{"name":"the Cholesterol Center, Medical Service, Long Beach (Calif) Department of Veterans Affairs Medical Center, and the University of California, Irvine."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(89)90118-5"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-7125(16)31425-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198308183090701"},{"key":"e_1_3_2_4_2","first-page":"1","volume":"12","year":"1983","journal-title":"Artery"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/353265a0"},{"key":"e_1_3_2_6_2","first-page":"369","volume":"3","year":"1979","journal-title":"Scand J Clin Lab Invest"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(85)90116-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113682"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.20.9607"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-117-8-646"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(86)80293-5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198711123172001"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199011083231901"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-7435(91)90006-P"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1016\/S0021-9258(18)89646-6","volume":"260","year":"1985","journal-title":"J Biol Chem"},{"key":"e_1_3_2_16_2","unstructured":"Kashyap ML. 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