{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T13:12:21Z","timestamp":1686143541242},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ATVB"],"published-print":{"date-parts":[[1997,11]]},"abstract":"<jats:p><jats:italic>Abstract<\/jats:italic>We report here that platelets adhere to cultured endothelial cells (EC) on exposure to the integrin \u03b21 activating monoclonal antibody (mAb) BV7. The effect of BV7 is exerted mostly on platelets rather than EC. BV7 does not induce platelet aggregation or 5-hydroxytyptamine (5-HT) release and does not increase platelet adhesion to matrix proteins. Another activating \u03b21 mAb, Lia1\/2, triggers an effect similar to BV7. Blocking antibodies to \u03b12 and \u03b21, but not to other integrin chains, are able to inhibit BV7-mediated adhesion. Moreover, the effect of BV7 requires active cellular metabolism and is not inhibited by platelet treatment with aspirin, by the PAF receptor antagonist BN50730, the phosphokinase C inhibitor staurosporin, or by the cAMP or cGMP enhancers prostaglandin E1 and sodium nitroprusside, respectively. Finally, BV7-mediated adhesion was enhanced by the endoperoxide analogue U46619. These data describe a novel mechanism of platelet adhesion to endothelial cells. This adhesion pathway appears to be mediated by \u03b12\u03b21-integrin on platelets and a still-undefined endothelial counter receptor.<\/jats:p>","DOI":"10.1161\/01.atv.17.11.2663","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"2663-2671","source":"Crossref","is-referenced-by-count":0,"title":["Triggering of \u03b21-Integrin Chain Induces Platelet Adhesion to Cultured Endothelium"],"prefix":"10.1161","volume":"17","author":[{"family":"Aldo Del Maschio","sequence":"first","affiliation":[{"name":"From the Laboratory of Vascular Biology and Human Immunology, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ine\u0301s","family":"Marti\u0301n-Padura","sequence":"additional","affiliation":[{"name":"From the Laboratory of Vascular Biology and Human Immunology, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Bernasconi","sequence":"additional","affiliation":[{"name":"From the Laboratory of Vascular Biology and Human Immunology, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabetta","family":"Dejana","sequence":"additional","affiliation":[{"name":"From the Laboratory of Vascular Biology and Human Immunology, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199007053230106"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1661303"},{"key":"e_1_3_2_3_2","first-page":"425","volume":"100","year":"1982","journal-title":"J Lab Clin Med."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/153.4.694"},{"key":"e_1_3_2_5_2","first-page":"2606","volume":"39","year":"1980","journal-title":"Fed Proc."},{"key":"e_1_3_2_6_2","first-page":"372","volume":"112","year":"1988","journal-title":"J Lab Clin Med."},{"key":"e_1_3_2_7_2","first-page":"H423","volume":"257","year":"1989","journal-title":"Am J Physiol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109197"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(87)80299-1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1993.tb13601.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(85)90013-1"},{"key":"e_1_3_2_12_2","first-page":"539","volume":"24","year":"1992","journal-title":"J Submicrosc Cytol Pathol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0026-2862(81)90005-4"},{"key":"e_1_3_2_14_2","first-page":"331","volume":"113","year":"1983","journal-title":"Am J Pathol."},{"key":"e_1_3_2_15_2","first-page":"33","volume":"145","year":"1994","journal-title":"Am J Pathol."},{"key":"e_1_3_2_16_2","first-page":"408","volume":"54","year":"1986","journal-title":"Lab Invest."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1253\/jcj.56.178"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1651046"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/359848a0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.1.146.146"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.16.7450"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"6124","DOI":"10.1016\/S0021-9258(17)37578-6","volume":"269","year":"1994","journal-title":"J Biol Chem."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.4049\/jimmunol.131.6.2895","volume":"131","year":"1983","journal-title":"J Immunol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1990.258.3.H870"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1648888"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(83)90027-9"},{"key":"e_1_3_2_27_2","first-page":"525","volume":"65","year":"1991","journal-title":"Lab Invest."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.3.479"},{"key":"e_1_3_2_29_2","first-page":"651","volume":"116","year":"1990","journal-title":"J Lab Clin Med."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.5.1917"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.3.803"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.4.1387"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.3.479"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.4.761"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830221213"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.4.1181.1181"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.3.659"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V82.4.1197.1197"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114309"},{"key":"e_1_3_2_40_2","first-page":"916","volume":"69","year":"1993","journal-title":"Thromb Haemostas."},{"key":"e_1_3_2_41_2","unstructured":"Mullane K. Neutrophil-platelet interactions and post-ischemic myocardial injury in: Schroer K Sinzinger H (editors): Prostaglandins in Clinical Research: Cardiovascular System. NY: Alan Liss; 1989;39\u201351."},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"4015","DOI":"10.4049\/jimmunol.143.12.4015","volume":"143","year":"1989","journal-title":"J Immunol."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.4.785"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.ATV.17.11.2663","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T12:34:12Z","timestamp":1686141252000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.17.11.2663"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,11]]},"references-count":43,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1997,11]]}},"alternative-id":["10.1161\/01.ATV.17.11.2663"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.17.11.2663","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,11]]}}}