{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T18:45:14Z","timestamp":1771008314199,"version":"3.50.1"},"reference-count":41,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ATVB"],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:p><jats:italic>Abstract<\/jats:italic>Triflavin, a 7.5-kD cysteine-rich polypeptide purified from<jats:italic>Trimeresurus favoviridis<\/jats:italic>snake venom, belongs to a family of Arg-Gly-Asp-(RGD)\u2013containing peptides, termed disintegrins. In this study, aggregating human platelets dose- dependently induced vasoconstriction in de-endothelialized isolated rat thoracic aortas. At 5\u00d710<jats:sup>7<\/jats:sup>cells per milliliter, platelets induced a peak tension averaging 65\u00b17.2% of the tension induced by phenylephrine (10 \u03bcmol\/L). The relative effectiveness of RGD-containing peptides (including venom peptides triflavin and trigramin, small RGD synthetic peptides Gly-Arg-Gly-Asp-Ser [GRGDS], Gly-Arg-Gly-Asp-Phe [GRGDF], and Gly-Arg-Gly-Asp-Ser-Pro-Lys [GRGDSPK]) was examined by testing the inhibitory effect on aggregating platelet-induced vasoconstriction in de-endothelialized aorta. Triflavin (1 \u03bcmol\/L) significantly inhibited the platelet-induced vasoconstriction, whereas neither trigramin (10 \u03bcmol\/L) nor small RGD peptides (2 mmol\/L) (ie, GRGDS, GRGDF, and GRGDSPK) showed any significant effect. The release of serotonin and the formation of thromboxane A<jats:sub>2<\/jats:sub>from aggregating platelets were both significantly inhibited by triflavin (2 \u03bcmol\/L), whereas trigramin and small RGD-containing peptides showed no significant effect. On scanning electron micrographs of de-endothelialized aorta, aggregating platelets adhered to the subendothelium, with loss of their discoid shape, to form irregular spheres with pseudopod extensions. Triflavin (2 \u03bcmol\/L) markedly reduced the adhesion of platelets to the subendothelium in the same aorta. Furthermore, RGD-containing peptides (including triflavin, trigramin, and small RGD-containing peptides) inhibited the adhesion of 10 \u03bcg\/mL collagen-activated platelets to extracellular matrices (ie, fibronectin, vitronectin, and von Willebrand factor). It is concluded that the marked ability of triflavin to inhibit aggregating platelet-induced vasoconstriction in de-endothelialized aorta compared with other RGD-containing peptides (including trigramin), may be due at least partly to triflavin\u2019s efficiently preventing the activation of platelets subsequent to inhibition of serotonin release and thromboxane A<jats:sub>2<\/jats:sub>formation. However, the different abilities of triflavin compared with other RGD-containing peptides was not related to the ability to inhibit adhesion of platelets to extracellular matrices. Therefore, from the results of this study, it appears that triflavin may be a useful therapeutic agent for the treatment of thromboembolism and its associated angiospasm.<\/jats:p>","DOI":"10.1161\/01.atv.17.12.3461","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"3461-3468","source":"Crossref","is-referenced-by-count":27,"title":["Triflavin Inhibits Platelet-Induced Vasoconstriction in De-endothelialized Aorta"],"prefix":"10.1161","volume":"17","author":[{"given":"J.-R.","family":"Sheu","sequence":"first","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Taipei Medical College (J.-R.S., W.C.H., C.H.S.), the Department of Pharmacology, National Defense Medical Center (M.H.Y., Y.M.L.), and the Institute of Pharmacology, Medical College, National Taiwan University (T.F.H.), Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. H.","family":"Yen","sequence":"additional","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Taipei Medical College (J.-R.S., W.C.H., C.H.S.), the Department of Pharmacology, National Defense Medical Center (M.H.Y., Y.M.L.), and the Institute of Pharmacology, Medical College, National Taiwan University (T.F.H.), Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. C.","family":"Hung","sequence":"additional","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Taipei Medical College (J.-R.S., W.C.H., C.H.S.), the Department of Pharmacology, National Defense Medical Center (M.H.Y., Y.M.L.), and the Institute of Pharmacology, Medical College, National Taiwan University (T.F.H.), Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. M.","family":"Lee","sequence":"additional","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Taipei Medical College (J.-R.S., W.C.H., C.H.S.), the Department of Pharmacology, National Defense Medical Center (M.H.Y., Y.M.L.), and the Institute of Pharmacology, Medical College, National Taiwan University (T.F.H.), Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. H.","family":"Su","sequence":"additional","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Taipei Medical College (J.-R.S., W.C.H., C.H.S.), the Department of Pharmacology, National Defense Medical Center (M.H.Y., Y.M.L.), and the Institute of Pharmacology, Medical College, National Taiwan University (T.F.H.), Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. F.","family":"Huang","sequence":"additional","affiliation":[{"name":"From the Graduate Institute of Medical Sciences, Taipei Medical College (J.-R.S., W.C.H., C.H.S.), the Department of Pharmacology, National Defense Medical Center (M.H.Y., Y.M.L.), and the Institute of Pharmacology, Medical College, National Taiwan University (T.F.H.), Taipei, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112606"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(91)90854-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199302000-00010"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.959827"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(93)90177-3"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6574604"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"19827","DOI":"10.1016\/S0021-9258(19)77710-2","volume":"263","year":"1988","journal-title":"J Biol Chem"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.20.8050"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"21550","DOI":"10.1016\/S0021-9258(20)88220-9","volume":"264","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(90)90229-9"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(91)90256-5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-195-43129B"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90233-9"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)61504-2"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"6529","DOI":"10.1016\/S0021-9258(18)83380-4","volume":"264","year":"1989","journal-title":"J Biol Chem"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"16157","DOI":"10.1016\/S0021-9258(18)47710-1","volume":"262","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00428a037"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2770351"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1646444"},{"key":"e_1_3_2_20_2","first-page":"329","volume":"109","year":"1991","journal-title":"J Biochem"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)92337-W"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V66.4.946.946"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/S0022-3565(25)23466-8","volume":"234","year":"1985","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00497016"},{"key":"e_1_3_2_25_2","first-page":"93","volume":"195","year":"1993","journal-title":"J Immunol Methods"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1649829"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(82)90346-6"},{"key":"e_1_3_2_28_2","first-page":"H1077","volume":"245","year":"1983","journal-title":"Am J Physiol"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.82.1.2364514"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1994.tb03721.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1648924"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(95)00530-7"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(94)90114-7"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.1.110.110"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V65.4.823.823"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1986.tb07552.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V60.3.663.663"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110448"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90101-1"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110946"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2420006"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.ATV.17.12.3461","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T07:51:47Z","timestamp":1741247507000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.17.12.3461"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1997,12]]}},"alternative-id":["10.1161\/01.ATV.17.12.3461"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.17.12.3461","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,12]]}}}