{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:36:54Z","timestamp":1791149814218,"version":"4.1.0"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ATVB"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p><jats:italic>Abstract<\/jats:italic>Within vessels, cyclooxygenase (COX) is expressed constitutively (COX-1) in endothelial cells where its production of prostacyclin is thought to contribute to the maintenance of vascular integrity. Recently, a novel isoform of COX, COX-2, has been described that is induced in animal arterial vessels after physical damage or exposure to proinflammatory cytokines. However, induction of COX-2 in human vessels has not been characterized. Moreover, the relative ability of arteries and veins to express COX-2 has not been addressed. Thus, we have compared the ability of segments of human saphenous vein and internal mammary artery, obtained from the same patient, to express COX-2 activity and mRNA after organ culture in the presence and absence of interleukin-1\u03b2. COX-2 metabolites, measured by radioimmunoassay, were released by both the internal mammary artery and saphenous vein in the following rank order: prostaglandin E<jats:sub>2<\/jats:sub>\u2265prostacyclin thromboxane A<jats:sub>2<\/jats:sub>. Inclusion of interleukin-1\u03b2 in the culture medium increased the release of prostanoids by the saphenous vein but not by the internal mammary artery. However, the selective COX-2 inhibitor NS-398 significantly attenuated prostacyclin release from both tissues. Northern blot analysis showed no detectable COX-2 mRNA in freshly prepared saphenous vein or internal mammary artery. In contrast, after 48 hours in organ culture, low levels of COX-2 mRNA were detected in both internal mammary artery and saphenous vein, an effect that was greatly increased by interleukin-1\u03b2. These observations show that COX-2 is induced in human saphenous vein and internal mammary artery and suggest that this may occur in humans after coronary artery bypass graft surgery. The induction of COX-2 and subsequent release of prostacyclin may represent an endogenous defense mechanism against endothelial damage incurred during surgical preparation of these vessels for bypass.<\/jats:p>","DOI":"10.1161\/01.atv.17.9.1644","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"1644-1648","source":"Crossref","is-referenced-by-count":42,"title":["Induction of Cyclooxygenase-2 in Human Saphenous Vein and Internal Mammary Artery"],"prefix":"10.1161","volume":"17","author":[{"given":"David","family":"Bishop-Bailey","sequence":"first","affiliation":[{"name":"From the Department of Applied Pharmacology (D.B.-B., S.W.L.) and Department of Thoracic Medicine (E.-B.H., R.N.), National Heart and Lung Institute, Imperial College of Science, Technology and Medicine; and Department of Cardiothoracic Surgery (J.R.P.) and Department of Anaesthetics and Critical Care Medicine (J.A.M.), Royal Brompton National Heart and Lung Hospital, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Pepper","sequence":"additional","affiliation":[{"name":"From the Department of Applied Pharmacology (D.B.-B., S.W.L.) and Department of Thoracic Medicine (E.-B.H., R.N.), National Heart and Lung Institute, Imperial College of Science, Technology and Medicine; and Department of Cardiothoracic Surgery (J.R.P.) and Department of Anaesthetics and Critical Care Medicine (J.A.M.), Royal Brompton National Heart and Lung Hospital, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.-B.","family":"Haddad","sequence":"additional","affiliation":[{"name":"From the Department of Applied Pharmacology (D.B.-B., S.W.L.) and Department of Thoracic Medicine (E.-B.H., R.N.), National Heart and Lung Institute, Imperial College of Science, Technology and Medicine; and Department of Cardiothoracic Surgery (J.R.P.) and Department of Anaesthetics and Critical Care Medicine (J.A.M.), Royal Brompton National Heart and Lung Hospital, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Newton","sequence":"additional","affiliation":[{"name":"From the Department of Applied Pharmacology (D.B.-B., S.W.L.) and Department of Thoracic Medicine (E.-B.H., R.N.), National Heart and Lung Institute, Imperial College of Science, Technology and Medicine; and Department of Cardiothoracic Surgery (J.R.P.) and Department of Anaesthetics and Critical Care Medicine (J.A.M.), Royal Brompton National Heart and Lung Hospital, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon W.","family":"Larkin","sequence":"additional","affiliation":[{"name":"From the Department of Applied Pharmacology (D.B.-B., S.W.L.) and Department of Thoracic Medicine (E.-B.H., R.N.), National Heart and Lung Institute, Imperial College of Science, Technology and Medicine; and Department of Cardiothoracic Surgery (J.R.P.) and Department of Anaesthetics and Critical Care Medicine (J.A.M.), Royal Brompton National Heart and Lung Hospital, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane A.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"From the Department of Applied Pharmacology (D.B.-B., S.W.L.) and Department of Thoracic Medicine (E.-B.H., R.N.), National Heart and Lung Institute, Imperial College of Science, Technology and Medicine; and Department of Cardiothoracic Surgery (J.R.P.) and Department of Anaesthetics and Critical Care Medicine (J.A.M.), Royal Brompton National Heart and Lung Hospital, London, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.eurheartj.a059476"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.71.4.382"},{"key":"e_1_3_2_3_2","first-page":"987","volume":"143","year":"1993","journal-title":"Am J Pathol"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/263663a0"},{"key":"e_1_3_2_5_2","first-page":"181","volume":"92","year":"1976","journal-title":"Br J Pharmacol"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.8.2800"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7827(86)90132-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.11693"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/atv91.14.7.8018656"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(95)00212-X"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110090"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.4049\/jimmunol.135.3.1872","volume":"135","year":"1985","journal-title":"J Immunol"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113346"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.16.7384"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118476"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.6.2046"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.8.3228"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb17048.x"},{"key":"e_1_3_2_19_2","first-page":"1401","volume":"137","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(94)00680-6"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4804(83)90130-0"},{"key":"e_1_3_2_23_2","first-page":"3285","volume":"285","year":"1983","journal-title":"J Biol Chem"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(91)90054-P"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-4975(93)91103-T"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1994.tb17093.x"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"16737","DOI":"10.1016\/S0021-9258(17)44821-6","volume":"265","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_3_2_28_2","first-page":"P136","volume":"115","year":"1995","journal-title":"Br J Pharmacol"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(94)90074-4"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2701"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.11.1644257"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.68.1.1702037"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(78)90177-6"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(77)90058-2"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041100103"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.ATV.17.9.1644","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T12:42:05Z","timestamp":1686141725000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.ATV.17.9.1644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":35,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1997,9]]}},"alternative-id":["10.1161\/01.ATV.17.9.1644"],"URL":"https:\/\/doi.org\/10.1161\/01.atv.17.9.1644","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}