{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:51:57Z","timestamp":1790923917462,"version":"4.1.0"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ATVB"],"published-print":{"date-parts":[[1999,3]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014Treatment of human aortic endothelial cells (EC) with minimally oxidized LDL (or minimally modified LDL, MM-LDL) produces a specific pattern of endothelial cell activation distinct from that produced by LPS, tumor necrosis factor-\u03b1, and interleukin-1, but similar to other agents that elevate cAMP. The current studies focus on the signal transduction pathways by which MM-LDL activates EC to bind monocytes. We now demonstrate that, in addition to an elevation of cAMP, lipoxygenase products are necessary for the MM-LDL response. Treatment of EC with inhibitors of the lipoxygenase pathway, 5,8,11,14-eicosatetraynoic acid (ETYA) or cinnamyl-3,4-dihydroxy-\u03b1-cyanocinnamate (CDC), blocked monocyte binding in MM-LDL-treated EC (MM-LDL=118\u00b113%; MM-LDL+ETYA=33\u00b14%; MM-LDL+CDC=23\u00b14% increase in monocyte binding) without reducing cAMP levels. To further investigate the role of the lipoxygenase pathway, cellular phospholipids were labeled with arachidonic acid. Treatment of cells for 4 hours with 50 to 100 \u03bcg\/mL MM-LDL, but not native LDL, caused a 60% increase in arachidonate release into the medium and increased the intracellular formation of 12(S)-HETE (\u2248100% increase). There was little 15(S)-HETE present, and no increase in its levels was observed. We demonstrated that 12(S)-HETE reversed the inhibitory effect of CDC. We also observed a 70% increase in the formation of 11,12-epoxyeicosatrienoic acid (11,12-EET) in cells treated with MM-LDL. To determine the mechanism of arachidonate release induced by MM-LDL, we examined the effects of MM-LDL on intracellular calcium levels. Treatment of EC with both native LDL and MM-LDL caused a rapid release of intracellular calcium from internal stores. However, several pieces of evidence suggest that calcium release alone does not explain the increased arachidonate release in MM-LDL-treated cells. The present studies suggest that products of 12-lipoxygenase play an important role in MM-LDL action on the induction of monocyte binding to EC.\n          <\/jats:p>","DOI":"10.1161\/01.atv.19.3.680","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T19:44:19Z","timestamp":1308339859000},"page":"680-686","source":"Crossref","is-referenced-by-count":47,"title":["Induction of Monocyte Binding to Endothelial Cells by MM-LDL"],"prefix":"10.1161","volume":"19","author":[{"given":"Henry M.","family":"Honda","sequence":"first","affiliation":[{"name":"From the Department of Medicine (Cardiology) (H.M.H., J.I.G., J.N.W., J.A.B.), Physiology (J.N.W.), and Pathology and Laboratory Medicine (N.L., M.F., J.A.B.), Cardiovascular Research Laboratory (H.M.H., J.I.G., J.N.W.), University of California at Los Angeles; and City of Hope National Medical Center (R.N., J.L.N.), Duarte, Calif."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Leitinger","sequence":"additional","affiliation":[{"name":"From the Department of Medicine (Cardiology) (H.M.H., J.I.G., J.N.W., J.A.B.), Physiology (J.N.W.), and Pathology and Laboratory Medicine (N.L., M.F., J.A.B.), Cardiovascular Research Laboratory (H.M.H., J.I.G., J.N.W.), University of California at Los Angeles; and City of Hope National Medical Center (R.N., J.L.N.), Duarte, Calif."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Frankel","sequence":"additional","affiliation":[{"name":"From the Department of Medicine (Cardiology) (H.M.H., J.I.G., J.N.W., J.A.B.), Physiology (J.N.W.), and Pathology and Laboratory Medicine (N.L., M.F., J.A.B.), Cardiovascular Research Laboratory (H.M.H., J.I.G., J.N.W.), University of California at Los Angeles; and City of Hope National Medical Center (R.N., J.L.N.), Duarte, Calif."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua I.","family":"Goldhaber","sequence":"additional","affiliation":[{"name":"From the Department of Medicine (Cardiology) (H.M.H., J.I.G., J.N.W., J.A.B.), Physiology (J.N.W.), and Pathology and Laboratory Medicine (N.L., M.F., J.A.B.), Cardiovascular Research Laboratory (H.M.H., J.I.G., J.N.W.), University of California at Los Angeles; and City of Hope National Medical Center (R.N., J.L.N.), Duarte, Calif."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rama","family":"Natarajan","sequence":"additional","affiliation":[{"name":"From the Department of Medicine (Cardiology) (H.M.H., J.I.G., J.N.W., J.A.B.), Physiology (J.N.W.), and Pathology and Laboratory Medicine (N.L., M.F., J.A.B.), Cardiovascular Research Laboratory (H.M.H., J.I.G., J.N.W.), University of California at Los Angeles; and City of Hope National Medical Center (R.N., J.L.N.), Duarte, Calif."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry L.","family":"Nadler","sequence":"additional","affiliation":[{"name":"From the Department of Medicine (Cardiology) (H.M.H., J.I.G., J.N.W., J.A.B.), Physiology (J.N.W.), and Pathology and Laboratory Medicine (N.L., M.F., J.A.B.), Cardiovascular Research Laboratory (H.M.H., J.I.G., J.N.W.), University of California at Los Angeles; and City of Hope National Medical Center (R.N., J.L.N.), Duarte, Calif."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James N.","family":"Weiss","sequence":"additional","affiliation":[{"name":"From the Department of Medicine (Cardiology) (H.M.H., J.I.G., J.N.W., J.A.B.), Physiology (J.N.W.), and Pathology and Laboratory Medicine (N.L., M.F., J.A.B.), Cardiovascular Research Laboratory (H.M.H., J.I.G., J.N.W.), University of California at Los Angeles; and City of Hope National Medical Center (R.N., J.L.N.), Duarte, Calif."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith A.","family":"Berliner","sequence":"additional","affiliation":[{"name":"From the Department of Medicine (Cardiology) (H.M.H., J.I.G., J.N.W., J.A.B.), Physiology (J.N.W.), and Pathology and Laboratory Medicine (N.L., M.F., J.A.B.), Cardiovascular Research Laboratory (H.M.H., J.I.G., J.N.W.), University of California at Los Angeles; and City of Hope National Medical Center (R.N., J.L.N.), Duarte, Calif."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116590"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114562"},{"key":"e_1_3_3_3_2","first-page":"458","volume":"14","year":"1994","journal-title":"Arterioscler Thromb"},{"key":"e_1_3_3_4_2","first-page":"427","volume":"70","year":"1990","journal-title":"Pathol Rev"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/92.2.241"},{"key":"e_1_3_3_6_2","unstructured":"Shih PT Elices MJ Fang ZT Ugarova TP Srahl D Territo MC Frank JS Kovach NL Cabanas C Berliner JA Vora DK. 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