{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:34:49Z","timestamp":1790516089302,"version":"4.1.0"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"18","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2002,10,29]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background\u2014<\/jats:italic>\n            <\/jats:bold>\n            Prostacyclin (PGI\n            <jats:sub>2<\/jats:sub>\n            ) is a short-lived endogenous inhibitor of platelet aggregation and a potent vasodilator and regulator of the growth of vascular smooth muscle cells. To study the role of PGI\n            <jats:sub>2<\/jats:sub>\n            in the vascular system in vivo, PGI\n            <jats:sub>2<\/jats:sub>\n            -deficient (PGID) mice were established by genetic disruption of the PGI\n            <jats:sub>2<\/jats:sub>\n            synthase gene.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            PGI\n            <jats:sub>2<\/jats:sub>\n            synthase\u2013null mice were generated by replacing the exons of PGI\n            <jats:sub>2<\/jats:sub>\n            synthase gene that encodes for the catalytic site of the enzyme with a neomycin resistance gene. In these mice, PGI\n            <jats:sub>2<\/jats:sub>\n            levels in the plasma, kidneys, and lungs were reduced, whereas thromboxane and prostaglandin E\n            <jats:sub>2<\/jats:sub>\n            levels became elevated. Blood pressure and the amounts of urea nitrogen and creatinine in plasma of the PGID mice were significantly higher than those of wild-type mice (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). They developed progressive morphological abnormalities in the kidneys, accompanied by atrophy, surface irregularity, fibrosis, cyst, arterial sclerosis, and hypertrophy of vessel walls. Thickening of the thoracic aortic media and adventitia were observed in aged PGID mice. Importantly, these phenotypes have not been reported in PGI\n            <jats:sub>2<\/jats:sub>\n            receptor\u2013deficient mice.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            PGI\n            <jats:sub>2<\/jats:sub>\n            deficiency resulted in the development of vascular disorders with the thickening of vascular walls and interstitial fibrosis, especially in mouse kidneys. The findings demonstrated in vivo that PGI\n            <jats:sub>2<\/jats:sub>\n            is important in the homeostasis of blood vessels. Our established PGID mice are useful for studies on the initiation and development of vascular diseases, such as ischemic renal disorders with arterial sclerosis and infarction, and also for studies on the novel signaling pathway of PGI\n            <jats:sub>2<\/jats:sub>\n            .\n          <\/jats:p>","DOI":"10.1161\/01.cir.0000034733.93020.bc","type":"journal-article","created":{"date-parts":[[2002,10,28]],"date-time":"2002-10-28T22:22:10Z","timestamp":1035843730000},"page":"2397-2403","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":106,"title":["Prostacyclin-Deficient Mice Develop Ischemic Renal Disorders, Including Nephrosclerosis and Renal Infarction"],"prefix":"10.1161","volume":"106","author":[{"given":"Chieko","family":"Yokoyama","sequence":"first","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Yabuki","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Shimonishi","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Wada","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihisa","family":"Hatae","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Ohkawara","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Takeda","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taroh","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaru","family":"Okabe","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., M.W., T.H., S.O., T.T.), Division of Microcirculatory Kinetics (M.W., T.T.), and Department of Social and Environmental Medicine (J.T.), Graduate School of Medicine, Research Institute for Microbial Diseases (T.K.), and Genome Information Research Center (M.O.), Osaka University, Suita, Osaka, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Tanabe","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, National Cardiovascular Center Research Institute (C.Y., T.Y., M.S., 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