{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T22:40:47Z","timestamp":1793400047897,"version":"4.5.0"},"reference-count":44,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2000,9,12]]},"abstract":"<jats:p>\n            <jats:italic>Background<\/jats:italic>\n            \u2014Excessive bleeding may complicate congenital cardiac defects. To explain the pathogenesis of this abnormality, we evaluated selected parameters of primary hemostasis in patients with aortic valve stenosis before and after corrective surgery.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results<\/jats:italic>\n            \u2014We examined shear-induced platelet aggregation with the filter aggregometer test and von Willebrand factor (vWF) structure by evaluating the multimeric distribution and extent of subunit proteolysis. The platelet count was reduced before corrective surgery, and shear-induced platelet aggregation was impaired. Moreover, vWF multimers of higher molecular mass were decreased, and proteolytic subunit fragments were increased. After correction of the cardiac defect, all of these parameters returned to normal.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            \u2014Alterations of vWF and platelet function may contribute to the bleeding diathesis in patients with aortic valve stenosis. Improvement after corrective surgery suggests that the passage of blood through a stenosed aortic valve may result in shear forces that induce vWF interaction with platelets in the circulation and, in turn, trigger platelet clearance, vWF degradation, and the impairment of primary hemostasis.\n          <\/jats:p>","DOI":"10.1161\/01.cir.102.11.1290","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:42:29Z","timestamp":1339461749000},"page":"1290-1295","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":126,"title":["Proteolysis of von Willebrand Factor and Shear Stress\u2013Induced Platelet Aggregation in Patients With Aortic Valve Stenosis"],"prefix":"10.1161","volume":"102","author":[{"given":"F. I.","family":"Pareti","sequence":"first","affiliation":[{"name":"From the Angelo Bianchi Bonomi Hemophilia and Thrombosis Center and Department of Internal Medicine (F.I.P., A.L., C.B.), IRCCS Maggiore Hospital, Milan; Chair of Cardiac Surgery (M.Z.), Fondazione Monzino, Milan; Division of Cardiac Surgery (A. Sala), Ospedale di Circolo, Fondazione Macchi, Varese; and the Departments of Blood Transfusion and Clinical Pathology (A. Steffan), IRCCS-CRO, Aviano, Italy; and the Departments of Molecular and Experimental Medicine and of Vascular Biology (Z.M.R.), The..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lattuada","sequence":"additional","affiliation":[{"name":"From the Angelo Bianchi Bonomi Hemophilia and Thrombosis Center and Department of Internal Medicine (F.I.P., A.L., C.B.), IRCCS Maggiore Hospital, Milan; Chair of Cardiac Surgery (M.Z.), Fondazione Monzino, Milan; Division of Cardiac Surgery (A. Sala), Ospedale di Circolo, Fondazione Macchi, Varese; and the Departments of Blood Transfusion and Clinical Pathology (A. Steffan), IRCCS-CRO, Aviano, Italy; and the Departments of Molecular and Experimental Medicine and of Vascular Biology (Z.M.R.), The..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Bressi","sequence":"additional","affiliation":[{"name":"From the Angelo Bianchi Bonomi Hemophilia and Thrombosis Center and Department of Internal Medicine (F.I.P., A.L., C.B.), IRCCS Maggiore Hospital, Milan; Chair of Cardiac Surgery (M.Z.), Fondazione Monzino, Milan; Division of Cardiac Surgery (A. Sala), Ospedale di Circolo, Fondazione Macchi, Varese; and the Departments of Blood Transfusion and Clinical Pathology (A. Steffan), IRCCS-CRO, Aviano, Italy; and the Departments of Molecular and Experimental Medicine and of Vascular Biology (Z.M.R.), The..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Zanobini","sequence":"additional","affiliation":[{"name":"From the Angelo Bianchi Bonomi Hemophilia and Thrombosis Center and Department of Internal Medicine (F.I.P., A.L., C.B.), IRCCS Maggiore Hospital, Milan; Chair of Cardiac Surgery (M.Z.), Fondazione Monzino, Milan; Division of Cardiac Surgery (A. Sala), Ospedale di Circolo, Fondazione Macchi, Varese; and the Departments of Blood Transfusion and Clinical Pathology (A. Steffan), IRCCS-CRO, Aviano, Italy; and the Departments of Molecular and Experimental Medicine and of Vascular Biology (Z.M.R.), The..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Sala","sequence":"additional","affiliation":[{"name":"From the Angelo Bianchi Bonomi Hemophilia and Thrombosis Center and Department of Internal Medicine (F.I.P., A.L., C.B.), IRCCS Maggiore Hospital, Milan; Chair of Cardiac Surgery (M.Z.), Fondazione Monzino, Milan; Division of Cardiac Surgery (A. Sala), Ospedale di Circolo, Fondazione Macchi, Varese; and the Departments of Blood Transfusion and Clinical Pathology (A. Steffan), IRCCS-CRO, Aviano, Italy; and the Departments of Molecular and Experimental Medicine and of Vascular Biology (Z.M.R.), The..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Steffan","sequence":"additional","affiliation":[{"name":"From the Angelo Bianchi Bonomi Hemophilia and Thrombosis Center and Department of Internal Medicine (F.I.P., A.L., C.B.), IRCCS Maggiore Hospital, Milan; Chair of Cardiac Surgery (M.Z.), Fondazione Monzino, Milan; Division of Cardiac Surgery (A. Sala), Ospedale di Circolo, Fondazione Macchi, Varese; and the Departments of Blood Transfusion and Clinical Pathology (A. Steffan), IRCCS-CRO, Aviano, Italy; and the Departments of Molecular and Experimental Medicine and of Vascular Biology (Z.M.R.), The..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. M.","family":"Ruggeri","sequence":"additional","affiliation":[{"name":"From the Angelo Bianchi Bonomi Hemophilia and Thrombosis Center and Department of Internal Medicine (F.I.P., A.L., C.B.), IRCCS Maggiore Hospital, Milan; Chair of Cardiac Surgery (M.Z.), Fondazione Monzino, Milan; Division of Cardiac Surgery (A. Sala), Ospedale di Circolo, Fondazione Macchi, Varese; and the Departments of Blood Transfusion and Clinical Pathology (A. Steffan), IRCCS-CRO, Aviano, Italy; and the Departments of Molecular and Experimental Medicine and of Vascular Biology (Z.M.R.), The..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9610(86)90465-4"},{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(10)62112-1"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-105-1-54"},{"key":"e_1_3_1_4_2","first-page":"275","volume":"2","year":"1976","unstructured":"Ramsay DM, Buist TAS, MacLeod DAD, et al. Persistent bleeding due to angiodysplasia of the gut in von Willebrand\u2019s disease. Lancet. 1976;2:275\u2013278.","journal-title":"Lancet"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92434-H"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V71.6.1648.1648"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V67.3.758.758"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109795"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.5.1419.1419"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1651576"},{"key":"e_1_3_1_11_2","unstructured":"Nichols WC Cooney KA Ginsburg D et al. von Willebrand disease. In: Loscalzo J Schafer AI eds. Thrombosis and Hemorrhage. Baltimore Md: Blackwell Scientific Publications; 1998:729\u2013755."},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112394"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.16.6306"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.10.4223.bloodjournal87104223"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.9.3097"},{"key":"e_1_3_1_16_2","unstructured":"Ruggeri ZM. Structure and biosynthesis of von Willebrand factor. In: Ruggeri ZM ed. von Willebrand Factor and the Mechanisms of Platelet Function. Berlin Germany: Springer-Verlag\/RG Landes Co; 1998."},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115376"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198005083021902"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V59.6.1272.1272"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V81.3.720.720"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V79.1.38.38"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1657634"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1996.401939.x"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(94)70088-5"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.10.4235.bloodjournal87104235"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.8.2939.bloodjournal8882939"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.40.23352"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI973"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112736"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.2.625.625"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1650297"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830250106"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112843"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.5.1354.1354"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1998.tb01065.x"},{"key":"e_1_3_1_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00359a015"},{"key":"e_1_3_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81607-4"},{"key":"e_1_3_1_38_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2516.1998.440661.x"},{"key":"e_1_3_1_39_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114775"},{"key":"e_1_3_1_40_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198310063091402"},{"key":"e_1_3_1_41_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.10.3018.3018"},{"key":"e_1_3_1_42_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1002658"},{"key":"e_1_3_1_43_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.2.454.454"},{"key":"e_1_3_1_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(92)90210-2"}],"container-title":["Circulation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.CIR.102.11.1290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T12:59:41Z","timestamp":1715518781000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.CIR.102.11.1290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9,12]]},"references-count":44,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2000,9,12]]}},"alternative-id":["10.1161\/01.CIR.102.11.1290"],"URL":"https:\/\/doi.org\/10.1161\/01.cir.102.11.1290","relation":{},"ISSN":["0009-7322","1524-4539"],"issn-type":[{"value":"0009-7322","type":"print"},{"value":"1524-4539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9,12]]},"assertion":[{"value":"1999-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-04-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-09-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}