{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T14:01:54Z","timestamp":1782309714720,"version":"3.54.5"},"reference-count":39,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2001,3,20]]},"abstract":"<jats:p>\n            <jats:italic>Background<\/jats:italic>\n            \u2014Activation of circulating monocytes in patients with acute coronary syndromes may reflect exposure to bacterial products or stimulation by cytokines such as IFN-\u03b3. IFN-\u03b3 induces phosphorylation and nuclear translocation of transcription factor STAT-1, which initiates a specific program of gene induction. To explore whether monocyte activation is IFN-\u03b3 driven, patients with unstable (UA) or stable angina (SA) were compared for nuclear translocation of STAT-1 complexes and upregulation of IFN-\u03b3\u2013inducible genes CD64 and IP-10.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results<\/jats:italic>\n            \u2014Peripheral blood mononuclear cells were stained for expression of CD64 on CD14\n            <jats:sup>+<\/jats:sup>\n            monocytes and analyzed by PCR for transcription of IP-10. Expression of CD64 was significantly increased in patients with UA. Monocytes from UA patients remained responsive to IFN-\u03b3 in vitro, with accelerated transcriptional competency of CD64. IP-10\u2013specific sequences were spontaneously detectable in 82% of the UA patients and 15% of SA patients (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.001). Most importantly, STAT-1 complexes were found in nuclear extracts prepared from freshly isolated monocytes of patients with UA, which provides compelling evidence for IFN-\u03b3 signaling in vivo.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            \u2014Monocytes from UA patients exhibit a molecular fingerprint of recent IFN-\u03b3 triggering, such as nuclear translocation of STAT-1 complexes and upregulation of IFN-\u03b3\u2013inducible genes CD64 and IP-10, which suggests that monocytes are activated, at least in part, by IFN-\u03b3. IFN-\u03b3 may derive from stimulated T lymphocytes, which implicates specific immune responses in the pathogenesis of acute coronary syndromes.\n          <\/jats:p>","DOI":"10.1161\/01.cir.103.11.1509","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:09:32Z","timestamp":1339459772000},"page":"1509-1514","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":73,"title":["Molecular Fingerprint of Interferon-\u03b3 Signaling in Unstable Angina"],"prefix":"10.1161","volume":"103","author":[{"given":"Giovanna","family":"Liuzzo","sequence":"first","affiliation":[{"name":"From the Department of Medicine, Mayo Clinic and Foundation, Rochester, Minn."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbe N.","family":"Vallejo","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Mayo Clinic and Foundation, Rochester, Minn."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L.","family":"Kopecky","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Mayo Clinic and Foundation, Rochester, Minn."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Frye","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Mayo Clinic and Foundation, Rochester, Minn."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Holmes","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Mayo Clinic and Foundation, Rochester, Minn."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u0308rg J.","family":"Goronzy","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Mayo Clinic and Foundation, Rochester, Minn."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia M.","family":"Weyand","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Mayo Clinic and Foundation, Rochester, Minn."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(99)00191-1"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-8703(99)70266-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8651(05)70074-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115747"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-199610000-00012"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.89.1.8281687"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.96.2.396"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.99.24.3103"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.90.4.1662"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.88.2.358"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(96)00367-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.94.5.874"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(97)00172-1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1614932"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00130-8"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199408183310701"},{"key":"e_1_3_2_17_2","first-page":"391","volume":"45","year":"2000","unstructured":"Ridker PM. 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