{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:53:20Z","timestamp":1790736800673,"version":"4.1.0"},"reference-count":25,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2001,2,6]]},"abstract":"<jats:p>\n            <jats:italic>Background<\/jats:italic>\n            \u2014After cardioversion of atrial fibrillation (AF), the contractile function of the atria is temporarily impaired. Although this has significant clinical implications, the underlying cellular mechanisms are poorly understood.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results<\/jats:italic>\n            \u2014Forty-nine consecutive patients submitted for mitral valve surgery were investigated. Twenty-three were in persistent AF (\u22653 months); the others were in sinus rhythm. Before extracorporal circulation, the right atrial appendage was excised. \u03b2-Adrenoceptors were quantified by radioligand binding, and G proteins were quantified by Western blot analysis. The isometric contractile response to Ca\n            <jats:sup>2+<\/jats:sup>\n            , isoproterenol, Bay K8644, and the postrest potentiation of contractile force were investigated in thin atrial trabeculae, which were also examined histologically. The contractile force of the atrial preparations obtained from AF patients was 75% less than that in preparations from patients in sinus rhythm. Also, the positive inotropic effect of isoproterenol was impaired, and Bay K8644 failed to increase atrial contractile force. In contrast, the response to extracellular Ca\n            <jats:sup>2+<\/jats:sup>\n            was maintained, and the postrest potentiation was preserved. \u03b2-Adrenoceptor density and G-protein expression were unchanged. Histological examination revealed 14% more myolysis in the atria of AF patients.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            \u2014After prolonged AF, atrial contractility was reduced by 75%. The impairment of \u03b2-adrenergic modulation of contractile force cannot be explained by downregulation of \u03b2-adrenoceptors or changes in G proteins. Dysfunction of the sarcoplasmic reticulum does not occur after prolonged AF. Failure of Bay K8644 to restore contractility suggests that the L-type Ca\n            <jats:sup>2+<\/jats:sup>\n            channel is responsible for the contractile dysfunction. The restoration of contractile force by high extracellular Ca\n            <jats:sup>2+<\/jats:sup>\n            shows that the contractile apparatus itself is nearly completely preserved after prolonged AF.\n          <\/jats:p>","DOI":"10.1161\/01.cir.103.5.691","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:09:32Z","timestamp":1339459772000},"page":"691-698","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":183,"title":["Cellular Mechanisms of Depressed Atrial Contractility in Patients With Chronic Atrial Fibrillation"],"prefix":"10.1161","volume":"103","author":[{"given":"Ulrich","family":"Schotten","sequence":"first","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannie","family":"Ausma","sequence":"additional","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Stellbrink","sequence":"additional","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingo","family":"Sabatschus","sequence":"additional","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Vogel","sequence":"additional","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Frechen","sequence":"additional","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedrich","family":"Schoendube","sequence":"additional","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Hanrath","sequence":"additional","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurits A.","family":"Allessie","sequence":"additional","affiliation":[{"name":"From the Cardiovascular Research Institute Maastricht (U.S., J.A., M.A.A.), University of Maastricht, Maastricht, the Netherlands, and the Departments of Cardiology (C.S., I.S., M.V., D.F., P.H.) and Thoracic and Cardiovascular Surgery (F.S.), University Hospital Aachen, Aachen, 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