{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:48:34Z","timestamp":1791179314934,"version":"4.1.0"},"reference-count":41,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2001,2,6]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    \u2014ADP plays a key role in hemostasis, acting through 2 platelet receptors: the P2Y\n                    <jats:sub>1<\/jats:sub>\n                    receptor and an unidentified P2 receptor, called P2cyc, coupled to adenylyl cyclase inhibition, which is the target of the antiplatelet drug clopidogrel. We showed that the P2Y\n                    <jats:sub>1<\/jats:sub>\n                    receptor is an essential cofactor in thrombotic states induced by intravenous infusion of collagen and epinephrine. The aim of the present study was to assess the role of this receptor in thrombin-dependent tissue factor\u2013induced thromboembolism.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods and Results<\/jats:italic>\n                    \u2014Human thromboplastin was injected intravenously into wild-type or P2Y\n                    <jats:sub>1<\/jats:sub>\n                    -deficient mice, and the effects on platelet count and mortality were determined and plasma thrombin\u2013antithrombin III (TAT) complexes were quantified. P2Y\n                    <jats:sub>1<\/jats:sub>\n                    -deficient mice were resistant to the thromboembolism induced by injection of thromboplastin. Whereas the platelet count decreased sharply in wild-type mice, there was no significant drop in platelets in P2Y\n                    <jats:sub>1<\/jats:sub>\n                    -knockout mice. The platelet consumption in wild-type mice was probably due to thrombin generation, because it was abolished by hirudin. Thromboplastin also led to a rise in TAT complexes in plasma, again reflecting thrombin formation. This effect, however, was less important in P2Y\n                    <jats:sub>1<\/jats:sub>\n                    -knockout mice than in wild-type mice, indicating that less thrombin was generated in the absence of P2Y\n                    <jats:sub>1<\/jats:sub>\n                    . Similar results were obtained after intravenous administration of\n                    <jats:italic>N<\/jats:italic>\n                    <jats:sup>6<\/jats:sup>\n                    -methyl-2\u2032-deoxyadenosine-3\u2032:5\u2032-bisphosphate, a selective antagonist of the P2Y\n                    <jats:sub>1<\/jats:sub>\n                    receptor, to wild-type mice.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusions<\/jats:italic>\n                    \u2014Our results demonstrate a role of the P2Y\n                    <jats:sub>1<\/jats:sub>\n                    receptor in thrombotic states involving thrombin generation and provide further evidence for the potential relevance of this receptor as a target for antithrombotic drugs.\n                  <\/jats:p>","DOI":"10.1161\/01.cir.103.5.718","type":"journal-article","created":{"date-parts":[[2012,6,11]],"date-time":"2012-06-11T20:09:32Z","timestamp":1339445372000},"page":"718-723","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":122,"title":["Key Role of the P2Y\n                    <sub>1<\/sub>\n                    Receptor in Tissue Factor\u2013Induced Thrombin-Dependent Acute Thromboembolism"],"prefix":"10.1161","volume":"103","author":[{"given":"Catherine","family":"Le\u0301on","sequence":"first","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U.311, Etablissement Franc\u0327ais du Sang\u2013Alsace, Strasbourg, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Freund","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U.311, Etablissement Franc\u0327ais du Sang\u2013Alsace, Strasbourg, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Ravanat","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U.311, Etablissement Franc\u0327ais du Sang\u2013Alsace, Strasbourg, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Baurand","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U.311, Etablissement Franc\u0327ais du Sang\u2013Alsace, Strasbourg, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Cazenave","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U.311, Etablissement Franc\u0327ais du Sang\u2013Alsace, Strasbourg, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gachet","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U.311, Etablissement Franc\u0327ais du Sang\u2013Alsace, Strasbourg, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","unstructured":"Walsh PN Schmaier AH. Platelet-coagulant protein interactions. In: Colman RW Hirsh J Marder VJ et al eds. Hemostasis and Thrombosis: Basic Principles and Clinical Practice. 3rd ed. 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Mouse antithrombotic assay: a simple method for the evaluation of antithrombotic agents in vivo: potentiation of antithrombotic activity by ethyl alcohol. 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N6-Methyl-2\u2032-deoxyadenosine-3\u2032-5\u2032-bisphosphate a potent and selective P2Y1 antagonist inhibits ADP-induced platelet aggregation in vitro and ex vivo and prolongs the bleeding time. Haematologica. 2000;85(suppl):90. 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