{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:50:17Z","timestamp":1761630617953,"version":"3.40.3"},"reference-count":42,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2001,2,27]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>\u2014P-selectin mediates leukocyte recruitment to activated platelets and endothelium through its high-affinity receptor P-selectin glycoprotein ligand-1 (PSGL-1). Platelet and leukocyte activation and binding have been reported after coronary angioplasty and were correlated with restenosis. We investigated the effect of a recombinant soluble PSGL-1 (rPSGL-Ig) on the adhesion of platelets and neutrophils and the development of restenosis after double arterial injury.<\/jats:p><jats:p><jats:italic>Methods and Results<\/jats:italic>\u2014Four weeks after angioplasty of both carotid arteries in pigs, a second angioplasty was performed at the same sites, 15 minutes after a single administration of vehicle or rPSGL-1 (1 mg\/kg IV). Animals were euthanized 1 hour, 4 hours, 1 week, or 4 weeks later. Adhesion of autologous<jats:sup>51<\/jats:sup>Cr-platelets and<jats:sup>111<\/jats:sup>In-neutrophils was quantified and histological\/morphometric analyses were performed. Although rPSGL-Ig did not affect adherence of these cells 1 hour after injury, it significantly reduced the adhesion of platelets (50% at 4 hours and 85% at 1 week) and neutrophils (50% at 4 hours and 78% at 1 week) to deeply injured arteries. At 4 weeks, the residual lumen was 63% larger in rPSGL-Ig\u2013treated arteries as compared with control arteries (6.1\u00b10.6 versus 3.8\u00b10.1 mm<jats:sup>2<\/jats:sup>;<jats:italic>P<\/jats:italic>&lt;0.002). The neointimal area was slightly reduced (0.5 in rPSGL-Ig versus 0.7 mm<jats:sup>2<\/jats:sup>in control). The ratio of the external elastic lamina of injured to uninjured reference segments was &gt;1 in treated arteries and &lt;1 in control arteries.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>\u2014P-selectin antagonism with rPSGL-Ig inhibits early platelet\/leukocyte adhesion on injured arteries and reduces restenosis through a positive impact on vascular remodeling. Hence, rPSGL-Ig may have potential in the prevention of restenosis.<\/jats:p>","DOI":"10.1161\/01.cir.103.8.1128","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:09:32Z","timestamp":1339459772000},"page":"1128-1134","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":51,"title":["Recombinant Soluble P-Selectin Glycoprotein Ligand-1-Ig Reduces Restenosis Through Inhibition of Platelet-Neutrophil Adhesion After Double Angioplasty in Swine"],"prefix":"10.1161","volume":"103","author":[{"given":"Jean-Guy","family":"Bienvenu","sequence":"first","affiliation":[{"name":"From the Research Center, Montreal Heart Institute (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), the University of Montreal (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), Montreal, Quebec, Canada; and Wyeth\/Genetics Institute (A.K., R.G.S.), Andover, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Franc\u0327ois","family":"Tanguay","sequence":"additional","affiliation":[{"name":"From the Research Center, Montreal Heart Institute (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), the University of Montreal (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), Montreal, Quebec, Canada; and Wyeth\/Genetics Institute (A.K., R.G.S.), Andover, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Franc\u0327ois","family":"The\u0301ore\u0302t","sequence":"additional","affiliation":[{"name":"From the Research Center, Montreal Heart Institute (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), the University of Montreal (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), Montreal, Quebec, Canada; and Wyeth\/Genetics Institute (A.K., R.G.S.), Andover, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anjali","family":"Kumar","sequence":"additional","affiliation":[{"name":"From the Research Center, Montreal Heart Institute (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), the University of Montreal (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), Montreal, Quebec, Canada; and Wyeth\/Genetics Institute (A.K., R.G.S.), Andover, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert G.","family":"Schaub","sequence":"additional","affiliation":[{"name":"From the Research Center, Montreal Heart Institute (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), the University of Montreal (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), Montreal, Quebec, Canada; and Wyeth\/Genetics Institute (A.K., R.G.S.), Andover, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yahye","family":"Merhi","sequence":"additional","affiliation":[{"name":"From the Research Center, Montreal Heart Institute (J.-G.B., J.-F. Tanguay, J-F. The\u0301ore\u0302t, Y.M.), the University of Montreal (J.-G.B., J.-F. Tanguay, J-F. 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