{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T01:26:52Z","timestamp":1789349212213,"version":"build-2803163510"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[1995,4]]},"abstract":"<jats:p>\n            <jats:italic>Background<\/jats:italic>\n            The induction of vascular cell adhesion molecule\u20131 (VCAM-1) and E-selectin by tumor necrosis factor\u2013\u03b1 (TNF) is mediated by mobilization of the transcription factor nuclear factor\u2013\u03baB (NF-\u03baB). Since salicylates have been reported to inhibit NF-\u03baB activation by preventing the degradation of its inhibitor I\u03baB, we studied a potential inhibition of this pathway by acetylsalicylate (aspirin) in human umbilical vein endothelial cells (HUVECs).\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results<\/jats:italic>\n            Gel-shift analyses demonstrated dose-dependent inhibition of TNF-induced NF-\u03baB mobilization by aspirin at concentrations ranging from 1 to 10 mmol\/L. Induction of VCAM-1 and E-selectin surface expression by TNF was dose-dependently reduced by aspirin over the same range, while induction of intercellular adhesion molecule\u20131 (ICAM-1) was hardly affected. Aspirin appeared to prevent VCAM-1 transcription, since it dose-dependently inhibited induction of VCAM-1 mRNA by TNF. As a functional consequence, adhesion of U937 monocytes to TNF-stimulated HUVECs was markedly reduced by aspirin due to suppression of VCAM-1 and E-selectin upregulation. These effects of aspirin were not related to the inhibition of cyclooxygenase activity, since indomethacin was ineffective.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            Our data suggest that aspirin inhibits NF-\u03baB mobilization, induction of VCAM-1 and E-selectin, and subsequent monocyte adhesion in endothelial cells stimulated by TNF, thereby providing an additional mechanism for therapeutic effects of aspirin.\n          <\/jats:p>","DOI":"10.1161\/01.cir.91.7.1914","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:34:42Z","timestamp":1339461282000},"page":"1914-1917","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":209,"title":["Aspirin Inhibits Nuclear Factor\u2013\u03baB Mobilization and Monocyte Adhesion in Stimulated Human Endothelial Cells"],"prefix":"10.1161","volume":"91","author":[{"given":"Christian","family":"Weber","sequence":"first","affiliation":[{"name":"From the Institut fu\u0308r Prophylaxe der Kreislaufkrankheiten, Ludwig-Maximilians-Universita\u0308t, Mu\u0308nchen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Erl","sequence":"additional","affiliation":[{"name":"From the Institut fu\u0308r Prophylaxe der Kreislaufkrankheiten, Ludwig-Maximilians-Universita\u0308t, Mu\u0308nchen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Pietsch","sequence":"additional","affiliation":[{"name":"From the Institut fu\u0308r Prophylaxe der Kreislaufkrankheiten, Ludwig-Maximilians-Universita\u0308t, Mu\u0308nchen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Weber","sequence":"additional","affiliation":[{"name":"From the Institut fu\u0308r Prophylaxe der Kreislaufkrankheiten, Ludwig-Maximilians-Universita\u0308t, Mu\u0308nchen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.54.4.363"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/362801a0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V77.10.2266.2266"},{"key":"e_1_3_2_4_2","first-page":"5","volume":"64","year":"1991","unstructured":"Libby P, Hansson G. 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