{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T19:11:31Z","timestamp":1789931491241,"version":"4.0.1"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[1995,11,15]]},"abstract":"<jats:p>\n            <jats:italic>Background<\/jats:italic>\n            Annexin V is a human phospholipid binding protein that binds to activated platelets in vitro. We sought to determine the potential of this agent for imaging intracardiac thrombi in swine.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results<\/jats:italic>\n            Left atrial thrombi were formed by crush injury. In initial nonimaging experiments using intravenous\n            <jats:sup>125<\/jats:sup>\n            I-labeled human annexin V, the mean thrombus\/whole blood ratio was 13.4\u00b14.8 for the entire thrombus using well counting of resected specimens (n=8). Using intravenously injected\n            <jats:sup>99m<\/jats:sup>\n            Tc-labeled human annexin V, the left atrial thrombus\/blood ratio by well counting was similar (14.2\u00b110.6 for the entire thrombus and 26.2\u00b114.9 for the peak section) (n=12). The ratio for a control protein,\n            <jats:sup>125<\/jats:sup>\n            I-ovalbumin, was only 1.0\u00b10.2.\n            <jats:sup>99m<\/jats:sup>\n            Tc tomographic imaging was positive (n=10) or equivocal (n=2) in all experiments with but negative in 10 controls without left atrial thrombi. By region-of-interest analysis of the tomographic images, the mean left atrial appendage\/blood ratio at 2 hours in animals with a thrombus was 3.90\u00b11.12 compared with 0.84\u00b10.10 in closed chest controls and 1.01\u00b10.23 in open chest controls (\n            <jats:italic>P<\/jats:italic>\n            &lt;.001).\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            We conclude that\n            <jats:sup>99m<\/jats:sup>\n            Tc-labeled human annexin V detects acute left atrial thrombi in vivo in swine. The combination of a new thrombus detection agent, annexin V, with a\n            <jats:sup>99m<\/jats:sup>\n            Tc label may allow in vivo imaging of thrombi in humans.\n          <\/jats:p>","DOI":"10.1161\/01.cir.92.10.3113","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:34:42Z","timestamp":1339461282000},"page":"3113-3121","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":65,"title":["Selective Uptake of Radiolabeled Annexin V on Acute Porcine Left Atrial Thrombi"],"prefix":"10.1161","volume":"92","author":[{"given":"John R.","family":"Stratton","sequence":"first","affiliation":[{"name":"From the Division of Cardiology, Department of Medicine (J.R.S., T.A.D., M.D.C.), the Department of Laboratory Medicine (J.F.T.), and the Division of Metabolism, Endocrinology and Nutrition (D.G.B.), Seattle VA Medical Center and University of Washington, and NeoRx Corporation (S.K., J.M.R.), Seattle, Wash."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy A.","family":"Dewhurst","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Department of Medicine (J.R.S., T.A.D., M.D.C.), the Department of Laboratory Medicine (J.F.T.), and the Division of Metabolism, Endocrinology and Nutrition (D.G.B.), Seattle VA Medical Center and University of Washington, and NeoRx Corporation (S.K., J.M.R.), Seattle, Wash."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhakar","family":"Kasina","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Department of Medicine (J.R.S., T.A.D., M.D.C.), the Department of Laboratory Medicine (J.F.T.), and the Division of Metabolism, Endocrinology and Nutrition (D.G.B.), Seattle VA Medical Center and University of Washington, and NeoRx Corporation (S.K., J.M.R.), Seattle, Wash."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Reno","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Department of Medicine (J.R.S., T.A.D., M.D.C.), the Department of Laboratory Medicine (J.F.T.), and the Division of Metabolism, Endocrinology and Nutrition (D.G.B.), Seattle VA Medical Center and University of Washington, and NeoRx Corporation (S.K., J.M.R.), Seattle, Wash."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel D.","family":"Cerqueira","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Department of Medicine (J.R.S., T.A.D., M.D.C.), the Department of Laboratory Medicine (J.F.T.), and the Division of Metabolism, Endocrinology and Nutrition (D.G.B.), Seattle VA Medical Center and University of Washington, and NeoRx Corporation (S.K., J.M.R.), Seattle, Wash."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis G.","family":"Baskin","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Department of Medicine (J.R.S., T.A.D., M.D.C.), the Department of Laboratory Medicine (J.F.T.), and the Division of Metabolism, Endocrinology and Nutrition (D.G.B.), Seattle VA Medical Center and University of Washington, and NeoRx Corporation (S.K., J.M.R.), Seattle, Wash."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan F.","family":"Tait","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Department of Medicine (J.R.S., T.A.D., M.D.C.), the Department of Laboratory Medicine (J.F.T.), and the Division of Metabolism, Endocrinology and Nutrition (D.G.B.), Seattle VA Medical Center and University of Washington, and NeoRx Corporation (S.K., J.M.R.), Seattle, Wash."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.85.1.1728477"},{"key":"e_1_3_2_2_2","first-page":"554","volume":"34","year":"1993","unstructured":"Knight LC. Scintigraphic methods for detecting vascular thrombus. J Nucl Med. 1993;34:554-561.","journal-title":"J Nucl Med"},{"key":"e_1_3_2_3_2","unstructured":"Stratton JR. Thrombosis imaging with indium-111 labeled platelets. In: Marcus ML Schelbert HR Skorton DJ Wolf DJ eds. Cardiac Imaging: Principles and Practice. New York NY: WB Saunders Co; 1991:1121-1134."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(83)90216-3"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)38177-8"},{"key":"e_1_3_2_6_2","first-page":"174","volume":"115","year":"1990","unstructured":"Flaherty MJ, West S, Heimark RL, Fujikawa K, Tait JF. Placental anticoagulant protein-I: measurement in extracellular fluids and cells of the hemostatic system. J Lab Clin Med. 1990;115:174-181.","journal-title":"J Lab Clin Med"},{"key":"e_1_3_2_7_2","unstructured":"Stryer L. Biochemistry. 3rd ed. New York NY: WH Freeman; 1988:284-287."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00391a053"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00417a011"},{"key":"e_1_3_2_10_2","unstructured":"Tait J Gibson D. Measurement of membrane phospholipid asymmetry in normal and sickle-cell erythrocytes by means of annexin V binding. J Lab Clin Med. In press."},{"key":"e_1_3_2_11_2","first-page":"1445","volume":"32","year":"1991","unstructured":"Kasina S, Rao TN, Srinivasan A, Sanderson JA, Fitzner JN, Reno JM, Beaumier PL, Fritzberg AR. Development and biologic evaluation of a kit for preformed chelate technetium-99m radiolabeling of an antibody Fab fragment using a diamide dimercaptide chelating agent. J Nucl Med. 1991;32:1445-1451.","journal-title":"J Nucl Med"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.11.4025"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.73.2.2935327"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(86)90260-2"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(91)90236-P"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1651585"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.85.1.1728460"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.156.2.4011916"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.173.1.2781002"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.162.2.3797675"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.2.708.708"},{"key":"e_1_3_2_22_2","unstructured":"Stratton JR Cerqueira MD Kohler T. Imaging arterial thrombosis in humans: comparison of technetium-99m labeled monoclonal antifibrin antibodies and indium-111 labeled platelets. J Nucl Med. In press."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.3.1036"},{"key":"e_1_3_2_24_2","first-page":"710","volume":"33","year":"1992","unstructured":"Knight LC, Abrams MJ, Schwartz DA, Hauser MM, Kollman M, Gaul FE, Rauh DA, Maurer AH. Preparation and preliminary evaluation of technetium-99m-labeled fragment E1 for thrombus imaging. J Nucl Med. 1992;33:710-715.","journal-title":"J Nucl Med"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.83.1.1702038"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.85.1.1728459"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198206243062502"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.66.6.6216026"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(83)90141-8"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.78.4.3168184"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)54228-9"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(89)90470-0"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.67.2.6848223"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.45.2.148"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(91)90705-E"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(86)80275-3"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.81.4.2317901"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.75.5.3568301"}],"container-title":["Circulation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.CIR.92.10.3113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T15:34:45Z","timestamp":1715528085000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.CIR.92.10.3113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,15]]},"references-count":38,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1995,11,15]]}},"alternative-id":["10.1161\/01.CIR.92.10.3113"],"URL":"https:\/\/doi.org\/10.1161\/01.cir.92.10.3113","relation":{},"ISSN":["0009-7322","1524-4539"],"issn-type":[{"value":"0009-7322","type":"print"},{"value":"1524-4539","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11,15]]},"assertion":[{"value":"1994-03-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1995-07-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1995-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}