{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T16:13:42Z","timestamp":1778688822093,"version":"3.51.4"},"reference-count":18,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[1997,7,15]]},"abstract":"<jats:p>\n            <jats:italic>Background<\/jats:italic>\n            Gene delivery of the thymidine kinase (tk) gene combined with ganciclovir (GCV) limits intimal hyperplasia after abrasion of normal arteries. However, the low efficiency of adenoviral-mediated gene transfer to atherosclerotic arteries has raised concerns about the applicability of this strategy to the prevention of restenosis.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results<\/jats:italic>\n            A replication-defective adenoviral vector expressing tk (Ad-RSVtk) demonstrated selective toxicity toward GCV-treated arterial smooth muscle cells, with oligonucleolytic cleavage suggesting apoptosis. In vivo, after demonstration of tk expression after Ad-RSVtk delivery, the combination of Ad-RSVtk followed by GCV was tested in a rabbit model of angioplasty of atheromatous iliac arteries. Angioplasty (8 atm, 20 minutes) was performed by use of a hydrogel balloon coated with Ad-RSVtk (4\u00d710\n            <jats:sup>9<\/jats:sup>\n            plaque forming units). GCV was infused (25 mg.kg\n            <jats:sup>\u22121<\/jats:sup>\n            IV BID) from days 2 through 7 after angioplasty in 8 of 12 rabbits. Four weeks later, morphometric analysis demonstrated a reduced intima-to-media ratio in the group receiving combination therapy compared with Ad-RSVtk alone (3.0\u00b11.2 versus 5.2\u00b10.5,\n            <jats:italic>P<\/jats:italic>\n            &lt;.018). GCV per se had no effect on intimal hyperplasia after arterial injury.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            In vitro, Ad-RSVtk demonstrates selective toxicity toward GCV-treated arterial smooth muscle cells involving apoptosis. In vivo, GCV conditions reduction of neointimal formation after percutaneous delivery of Ad-RSVtk during angioplasty of atheromatous arteries.\n          <\/jats:p>","DOI":"10.1161\/01.cir.96.2.408","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:41:02Z","timestamp":1339461662000},"page":"408-411","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":60,"title":["Reduction of Restenosis After Angioplasty in an Atheromatous Rabbit Model by Suicide Gene Therapy"],"prefix":"10.1161","volume":"96","author":[{"given":"P. Gabriel","family":"Steg","sequence":"first","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ouafae","family":"Tahlil","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Aubailly","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Michel","family":"Caillaud","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Franc\u0327ois","family":"Dedieu","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Berthelot","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aude","family":"Le Roux","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Feldman","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Perricaudet","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrice","family":"Dene\u0300fle","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier","family":"Branellec","sequence":"additional","affiliation":[{"name":"From INSERM U-460, Faculte\u0301 Xavier Bichat, Paris, France (P.G.S., O.T., L.F.); Rho\u0302ne Poulenc Rorer-Gencell, France (N.A., J.-M.C., J.-F.D., K.B., P.D., D.B.); and UA 1301 CNRS, Institut Gustave Roussy, Villejuif, France (A.Le R., M.P.)."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(85)80174-1"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(92)90476-4"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1157"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8047883"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10732"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118770"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117968"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117938"},{"key":"e_1_3_2_9_2","first-page":"709","volume":"3","year":"1991","unstructured":"Plautz G, Nabel EJ, Nabel GJ. 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