{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:46:57Z","timestamp":1791420417451,"version":"4.3.3"},"reference-count":39,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[1997,10,7]]},"abstract":"<jats:p>\n            <jats:italic>Background<\/jats:italic>\n            The procoagulant effect of anionic phospholipid may play a major role in the development of arterial thrombosis.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results<\/jats:italic>\n            Annexin V, a calcium-dependent anionic-phospholipid\u2013binding protein, was expressed and isolated from\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and its antithrombotic effect examined in a rabbit carotid artery thrombosis model. A partially occlusive thrombus was formed in the left carotid artery by application of electric current to produce an \u224850% occlusion of the lumen. After the current was discontinued, flow ceased completely within 42\u00b112 minutes (n=6) because of continuing platelet\/fibrin thrombus formation. When annexin V was given at doses of 2.8 to 16.6 \u03bcg \u00b7 kg\n            <jats:sup>\u22121<\/jats:sup>\n            \u00b7 min\n            <jats:sup>\u22121<\/jats:sup>\n            for a period of 180 minutes, starting at the time the current was stopped, there was a dose-dependent inhibition of thrombus formation. At a dose of 5.6 \u03bcg \u00b7 kg\n            <jats:sup>\u22121<\/jats:sup>\n            \u00b7 min\n            <jats:sup>\u22121<\/jats:sup>\n            , blood flow remained patent throughout the infusion and for an additional 60 minutes after the infusion was stopped. In addition, there was a decrease in thrombus weight (16\u00b17.4 versus 2.0\u00b11.0 g),\n            <jats:sup>125<\/jats:sup>\n            I-fibrin deposition (\u224845% reduction,\n            <jats:italic>P<\/jats:italic>\n            &lt;.001), and\n            <jats:sup>111<\/jats:sup>\n            In-labeled platelet accumulation (\u224843% reduction,\n            <jats:italic>P<\/jats:italic>\n            &lt;.001). Prior mixing of annexin V with phosphatidylserine micelles abolished the antithrombotic effect of annexin V, whereas mixing with phosphatidylcholine micelles had no effect. The antithrombotic effect of annexin V was not associated with bleeding tendency, as judged by the amount of blood absorbed in a gauze pad placed in a surgical incision extending to the muscle tissue and by the standard template bleeding time.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            These observations support a potentially important role for anionic phospholipid exposure in platelets in arterial thrombosis, and inhibition of this activity could be a novel target for therapy in coronary thrombosis and stroke and after angioplasty.\n          <\/jats:p>","DOI":"10.1161\/01.cir.96.7.2339","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:41:02Z","timestamp":1339461662000},"page":"2339-2347","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":69,"title":["Inhibition of Arterial Thrombosis by Recombinant Annexin V in a Rabbit Carotid Artery Injury Model"],"prefix":"10.1161","volume":"96","author":[{"given":"Perumal","family":"Thiagarajan","sequence":"first","affiliation":[{"name":"From the Divisions of Hematology and Cardiology, Department of Internal Medicine, University of Texas Health Science Center, Houston."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude R.","family":"Benedict","sequence":"additional","affiliation":[{"name":"From the Divisions of Hematology and Cardiology, Department of Internal Medicine, University of Texas Health Science Center, Houston."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4439(94)90086-8"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-2994-1_15"},{"key":"e_1_3_2_3_2","first-page":"47","volume":"48","year":"1979","unstructured":"Op den Camp JAF. 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