{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T15:32:03Z","timestamp":1782228723252,"version":"3.54.5"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"14","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[1999,4,13]]},"abstract":"<jats:p>\n            <jats:italic>Background<\/jats:italic>\n            \u2014It is a widely held view that congenital heart block (CHB) is caused by the transplacental transfer of maternal autoantibodies (anti-SSA\/Ro and\/or anti-SSB\/La) into the fetal circulation. To test this hypothesis and to reproduce human CHB, an experimental mouse model (\n            <jats:italic>BALB\/c)<\/jats:italic>\n            was developed by passive transfer of human autoantibodies into pregnant mice.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Methods and Results<\/jats:italic>\n            \u2014Timed pregnant mice (n=54) were injected with a single intravenous bolus of purified IgG containing human anti-SSA\/Ro and anti-SSB\/La antibodies from mothers of children with CHB. To parallel the \u201cwindow period\u201d of susceptibility to CHB in humans, 3 groups of mice were used: 8, 11, and 16 days of gestation. Within each group, we tested 10, 25, 50, and 100 \u03bcg of IgG. At delivery, ECGs were recorded and analyzed for conduction abnormalities. Bradycardia and PR interval were significantly increased in 8-, 11-, and 16-day gestational groups when compared with controls (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). QRS duration was not significantly different between all groups. Antibody levels measured by ELISA in both mothers and their offspring confirmed the transplacental transfer of the human antibodies to the pups.\n          <\/jats:p>\n          <jats:p>\n            <jats:italic>Conclusions<\/jats:italic>\n            \u2014The passive transfer model demonstrated bradycardia, first-degree but not complete atrioventricular block in pups. The greater percentage and degree of bradycardia and PR prolongation in the 11-day mouse group correlates with the \u201cwindow period\u201d of susceptibility observed in humans. The high incidence of bradycardia suggests possible sinoatrial node involvement. All together, these data provide relevant insights into the pathogenesis of CHB.\n          <\/jats:p>","DOI":"10.1161\/01.cir.99.14.1914","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:42:29Z","timestamp":1339461749000},"page":"1914-1918","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":72,"title":["Electrocardiographic Abnormalities in a Murine Model Injected With IgG From Mothers of Children With Congenital Heart Block"],"prefix":"10.1161","volume":"99","author":[{"given":"Jay A.","family":"Mazel","sequence":"first","affiliation":[{"name":"From the Cardiology Division, Veterans Administration Medical Center, and SUNY Health Science Center, Brooklyn, NY (N.E.-S., M.B.); the Cardiovascular Medicine Section, Yale New Haven Hospital, New Haven, Conn (J.A.M.); and the Department of Rheumatology, Hospital of Joint Diseases, New York University School of Medicine, New York, NY (J.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabil","family":"El-Sherif","sequence":"additional","affiliation":[{"name":"From the Cardiology Division, Veterans Administration Medical Center, and SUNY Health Science Center, Brooklyn, NY (N.E.-S., M.B.); the Cardiovascular Medicine Section, Yale New Haven Hospital, New Haven, Conn (J.A.M.); and the Department of Rheumatology, Hospital of Joint Diseases, New York University School of Medicine, New York, NY (J.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill","family":"Buyon","sequence":"additional","affiliation":[{"name":"From the Cardiology Division, Veterans Administration Medical Center, and SUNY Health Science Center, Brooklyn, NY (N.E.-S., M.B.); the Cardiovascular Medicine Section, Yale New Haven Hospital, New Haven, Conn (J.A.M.); and the Department of Rheumatology, Hospital of Joint Diseases, New York University School of Medicine, New York, NY (J.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Boutjdir","sequence":"additional","affiliation":[{"name":"From the Cardiology Division, Veterans Administration Medical Center, and SUNY Health Science Center, Brooklyn, NY (N.E.-S., M.B.); the Cardiovascular Medicine Section, Yale New Haven Hospital, New Haven, Conn (J.A.M.); and the Department of Rheumatology, Hospital of Joint Diseases, New York University School of Medicine, New York, NY (J.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","first-page":"467","volume":"4","year":"1901","unstructured":"Morquio L. 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