{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:32:54Z","timestamp":1773419574787,"version":"3.50.1"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9_supplement","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2005,8,30]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background\u2014<\/jats:italic>\n            <\/jats:bold>\n            Patients who undergo mechanical support with a left ventricular assist device (LVAD) exhibit reverse remodeling and in some cases recover from heart failure. We have developed a combination therapy using LVAD support combined with pharmacological therapy to maximize reverse remodeling, followed by the \u03b2\n            <jats:sub>2<\/jats:sub>\n            adrenergic agonist clenbuterol. We recently found that clenbuterol induces insulin-like growth factor I (IGF-I) in cardiac myocytes in vitro. The purpose of this study is to examine IGF-I expression in recovery patients after combination therapy.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            Myocardial mRNA levels were determined by real-time quantitative polymerase chain reaction in 12 recovery patients (at LVAD implantation, explantation, and 1 year after explantation). IGF-I mRNA was elevated at the time of LVAD explantation relative to donors, with 2 groups distinguishable: Those with low IGF-I mRNA at implantation who showed significant increase during recovery and those with high IGF-I mRNA at implantation who remained high. Levels returned to normal by 1 year after explantation. Microarray analysis of implantation and explantation samples of recovery patients further revealed elevated IGF-II and IGF binding proteins IGFBP4 and IGFBP6. IGF-I levels correlated with stromal cell-derived factor mRNA measured both in LVAD patients and in a wider cohort of heart failure patients.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            The data suggest involvement of elevated myocardial IGF-I mRNA in recovery. IGF-I may act to limit atrophy and apoptosis during reverse remodeling and to promote repair and regeneration in concert with stromal cell derived factor.\n          <\/jats:p>","DOI":"10.1161\/01.circulationaha.105.525873","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T22:28:22Z","timestamp":1625264902000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":39,"title":["Myocardial Insulin-Like Growth Factor-I Gene Expression During Recovery From Heart Failure After Combined Left Ventricular Assist Device and Clenbuterol Therapy"],"prefix":"10.1161","volume":"112","author":[{"given":"Paul J. R.","family":"Barton","sequence":"first","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leanne E.","family":"Felkin","sequence":"additional","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma J.","family":"Birks","sequence":"additional","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin E.","family":"Cullen","sequence":"additional","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas R.","family":"Banner","sequence":"additional","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Grindle","sequence":"additional","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Hall","sequence":"additional","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie W.","family":"Miller","sequence":"additional","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdi H.","family":"Yacoub","sequence":"additional","affiliation":[{"name":"From the National Heart and Lung Institute, Imperial College London, Heart Science Centre, London (P.J.R.B., L.E.F., M.E.C., M.H.Y.), and Royal Brompton and Harefield NHS Trust, Harefield, Middlesex (E.J.B., N.R.B.), UK; and Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis (S.G., J.L.H., L.W.M.)."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(96)00437-7"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.98.22.2383"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1053\/euhj.2001.2613"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/146837601317072185"},{"key":"e_1_3_2_5_2","first-page":"S2995","volume":"106","year":"2002","unstructured":"Yacoub M, Tansley P, Birks E, Hipkin M, Hardy J, Bowles C, Banner N, Khaghani A. 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