{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T15:09:29Z","timestamp":1779289769227,"version":"3.51.4"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:p>\n            Nitric oxide (NO) plays an important role in the control of vascular tone. Traditionally, its vasorelaxant activity has been attributed to the free radical form of NO (NO\n            <jats:sup>\u2022<\/jats:sup>\n            ), yet the reduced form of NO (NO\n            <jats:sup>\u2212<\/jats:sup>\n            ) is also produced endogenously and is a potent vasodilator of large conduit arteries. The effects of NO\n            <jats:sup>\u2212<\/jats:sup>\n            in the resistance vasculature remain unknown. This study examines the activity of NO\n            <jats:sup>\u2212<\/jats:sup>\n            in rat small isolated mesenteric resistance-like arteries and characterizes its mechanism(s) of action. With the use of standard myographic techniques, the vasorelaxant properties of NO\n            <jats:sup>\u2022<\/jats:sup>\n            (NO gas solution), NO\n            <jats:sup>\u2212<\/jats:sup>\n            (Angeli\u2019s salt), and the NO donor sodium nitroprusside were compared. Relaxation responses to Angeli\u2019s salt (pEC\n            <jats:sub>50<\/jats:sub>\n            =7.51\u00b10.13, R\n            <jats:sub>max<\/jats:sub>\n            =95.5\u00b11.5%) were unchanged in the presence of carboxy-PTIO (NO\n            <jats:sup>\u2022<\/jats:sup>\n            scavenger) but those to NO\n            <jats:sup>\u2022<\/jats:sup>\n            and sodium nitroprusside were inhibited.\n            <jats:sc>l<\/jats:sc>\n            -Cysteine (NO\n            <jats:sup>\u2212<\/jats:sup>\n            scavenger) decreased the sensitivity to Angeli\u2019s salt (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.01) and sodium nitroprusside (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.01) but not to NO\n            <jats:sup>\u2022<\/jats:sup>\n            . The soluble guanylate cyclase inhibitor ODQ (3 and 10 \u03bcmol\/L) concentration-dependently inhibited relaxation responses to Angeli\u2019s salt (41.0\u00b16.0% versus control 93.4\u00b11.9% at 10 \u03bcmol\/L). The voltage-dependent K\n            <jats:sup>+<\/jats:sup>\n            channel inhibitor 4-aminopyridine (1 mmol\/L) caused a 9-fold (\n            <jats:italic>P<\/jats:italic>\n            &lt;0.01) decrease in sensitivity to Angeli\u2019s salt, whereas glibenclamide, iberiotoxin, charybdotoxin, and apamin were without effect. In combination, ODQ and 4-aminopyridine abolished the response to Angeli\u2019s salt. In conclusion, NO\n            <jats:sup>\u2212<\/jats:sup>\n            functions as a potent vasodilator of resistance arteries, mediating its response independently of NO\n            <jats:sup>\u2022<\/jats:sup>\n            and through the activation of soluble guanylate cyclase and voltage-dependent K\n            <jats:sup>+<\/jats:sup>\n            channels. NO\n            <jats:sup>\u2212<\/jats:sup>\n            donors may represent a novel class of nitrovasodilator relevant for the treatment of cardiovascular disorders such as angina.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.0000072010.54901.de","type":"journal-article","created":{"date-parts":[[2003,5,13]],"date-time":"2003-05-13T05:39:36Z","timestamp":1052804376000},"page":"1301-1307","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":135,"title":["NO\n            <sup>\u2212<\/sup>\n            Activates Soluble Guanylate Cyclase and K\n            <sub>v<\/sub>\n            Channels to Vasodilate Resistance Arteries"],"prefix":"10.1161","volume":"41","author":[{"given":"Jennifer C.","family":"Irvine","sequence":"first","affiliation":[{"name":"From the Department of Pharmacology, Monash University, Melbourne, Victoria, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne L.","family":"Favaloro","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Monash University, Melbourne, Victoria, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara K.","family":"Kemp-Harper","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology, Monash University, Melbourne, Victoria, Australia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","first-page":"109","volume":"43","year":"1991","unstructured":"Moncada S, Palmer RM, Higgs EA. 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Potent and selective inhibition of nitric oxide-sensitive guanylyl cyclase by 1H-[1,2,4]oxodiazolo[4,3-a]quinoxalin-1-one. Mol Pharmacol. 1995; 48: 184\u2013188.","journal-title":"Mol Pharmacol"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(97)01393-0"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.277.5.G1055"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/368850a0"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701940"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704127"},{"key":"e_1_3_2_34_2","first-page":"H904","volume":"272","year":"1997","unstructured":"Zhao YJ, Wang J, Rubin LJ, Yuan XJ. Inhibition of Kv and KCa channels antagonizes NO-induced relaxation in pulmonary artery. Am J Physiol. 1997; 272: H904\u2013H912.","journal-title":"Am J Physiol"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703588"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.1.C299"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199802000-00010"}],"container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.HYP.0000072010.54901.DE","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T16:44:54Z","timestamp":1715532294000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.0000072010.54901.DE"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,6]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2003,6]]}},"alternative-id":["10.1161\/01.HYP.0000072010.54901.DE"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.0000072010.54901.de","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,6]]},"assertion":[{"value":"2003-01-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-05-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}