{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:07:19Z","timestamp":1791173239781,"version":"4.1.0"},"reference-count":51,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p>\n            Recent studies suggested that type 2 angiotensin receptor (AT2R) could contribute to regulation of blood pressure and\/or vascular remodeling. A key question relates to the effects of potential modulators of vascular AT2R expression. In the present work, we evaluated if high salt intake (70 mmol\/L NaCl in drinking water) could modulate rat mesenteric artery AT2R function and expression. Angiotensin II dose-response curves were studied in rat perfused pressurized small-diameter arteries in the presence of losartan (AT1R antagonist). Arteries were precontracted with phenylephrine, yielding \u224830% decrease in resting diameter. AT2R activation by angiotensin-induced dose-dependent relaxation of precontracted arteries (60.1\u00b19.1% of phenylephrine-induced contraction,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). In contrast, AT2R-dependent relaxation was not observed in arteries obtained from rats on high-salt diet. Semi-quantitative reverse-transcription polymerase chain reaction experiments demonstrated reduced amount of AT2R mRNA in arteries of rats on high-salt diet (65.5\u00b17.5% of control levels,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). Western blot studies demonstrated decreased AT2R in mesenteric artery protein fractions of high-salt diet rats (60.0\u00b118.0 of control levels,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). In a second set of experiments, adrenalectomy (4 days) blunted AT2R-mediated vasorelaxation and decreased AT2R mRNA (72.0\u00b111.0% of control levels,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). AT2R abundance in protein fractions of mesenteric arteries of ADX rats was also diminished (64.0\u00b113% of control levels,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.05). Both, AT2R mRNA and protein downregulation were prevented by mineralocorticoid replacement therapy. Finally, physiological concentrations of aldosterone caused a dose-dependent increase in AT2R mRNA of small diameter mesenteric artery explants. The results are consistent with aldosterone-mediated upregulation AT2R.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.0000161990.98383.ad","type":"journal-article","created":{"date-parts":[[2005,4,5]],"date-time":"2005-04-05T00:30:38Z","timestamp":1112661038000},"page":"853-859","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":28,"title":["High-Salt Diet Inhibits Expression of Angiotensin Type 2 Receptor in Resistance Arteries"],"prefix":"10.1161","volume":"45","author":[{"given":"Magdalena","family":"Gonzalez","sequence":"first","affiliation":[{"name":"From the Laboratory of Molecular and Integrative Physiology, Faculty of Medicine, University Los Andes, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Lobos","sequence":"additional","affiliation":[{"name":"From the Laboratory of Molecular and Integrative Physiology, Faculty of Medicine, University Los Andes, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Castillo","sequence":"additional","affiliation":[{"name":"From the Laboratory of Molecular and Integrative Physiology, Faculty of Medicine, University Los Andes, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorna","family":"Galleguillos","sequence":"additional","affiliation":[{"name":"From the Laboratory of Molecular and Integrative Physiology, Faculty of Medicine, University Los Andes, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandy C.","family":"Lopez","sequence":"additional","affiliation":[{"name":"From the Laboratory of Molecular and Integrative Physiology, Faculty of Medicine, University Los Andes, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Michea","sequence":"additional","affiliation":[{"name":"From the Laboratory of Molecular and Integrative Physiology, Faculty of Medicine, University Los Andes, Chile."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)90700-U"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74499-8"},{"key":"e_1_3_2_3_2","first-page":"S30","volume":"10","year":"1999","unstructured":"Ardaillou R. 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