{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:29:07Z","timestamp":1778275747214,"version":"3.51.4"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:p>\n                    Intracellular calcium and cAMP are the 2 second messengers that regulate renin release; cAMP stimulates renin release from juxtaglomerular (JG) cells, whereas increased intracellular calcium inhibits it. We hypothesized that decreased intracellular calcium acts by activating calcium-inhibitable isoforms of adenylyl cyclase, increasing cAMP, and stimulating renin secretion. We used a primary culture of JG cells isolated from C-57\/B6 mice. Cells were plated to a density of 70% in serum-free medium and incubated for 2 hours with or without 100 \u03bcmol\/L of the cytosolic calcium chelator 5\u20325-dimethyl-1,2-bis-(2-aminophenoxy)-ethane-N,N,N\u2032,N\u2032-tetra-acetic acid (BAPTA-AM) to decrease intracellular calcium. JG cell cAMP content and renin release were determined by radioimmunoassay. Intracellular cAMP content was 4.04\u00b10.92 pM\/mL per milligram of protein, and it increased by125\u00b133% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.01) with BAPTA-AM. Basal renin was 1.28\u00b10.40 \u03bcg of angiotensin I per milliliter per hour per milligram of protein, and BAPTA-AM increased it by 182\u00b162% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.025). Western blots using an antibody that recognizes adenylyl cyclase types V and VI yielded a characteristic band of \u2248135 kDa. When primary cultures of isolated JG cells were tested for the calcium-inhibitable isoforms of adenylyl cyclase, they showed intense focal cytoplasmic staining. Cells stained for both renin and adenylyl cyclase V\/VI showed colocalization in the cytoplasm, primarily on the granules. An adenylyl cyclase inhibitor (SQ 22,536) completely blocked BAPTA-AM\u2013stimulated renin release and JG cell cAMP content. We conclude that calcium-inhibitable isoform(s) of adenylyl cyclase (types V and\/or VI) exist within the JG cell. Thus, decreased intracellular calcium stimulates adenylyl cyclase, resulting in cAMP synthesis and, consequently, renin release.\n                  <\/jats:p>","DOI":"10.1161\/01.hyp.0000250708.04205.d4","type":"journal-article","created":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T20:54:14Z","timestamp":1162846454000},"page":"162-169","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":72,"title":["Decreased Intracellular Calcium Stimulates Renin Release via Calcium-Inhibitable Adenylyl Cyclase"],"prefix":"10.1161","volume":"49","author":[{"given":"M. Cecilia","family":"Ortiz-Capisano","sequence":"first","affiliation":[{"name":"From the Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo A.","family":"Ortiz","sequence":"additional","affiliation":[{"name":"From the Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Harding","sequence":"additional","affiliation":[{"name":"From the Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey L.","family":"Garvin","sequence":"additional","affiliation":[{"name":"From the Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William H.","family":"Beierwaltes","sequence":"additional","affiliation":[{"name":"From the Hypertension and Vascular Research Division, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","first-page":"F175","volume":"249","year":"1985","unstructured":"Churchill PC. 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