{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T22:12:40Z","timestamp":1769724760471,"version":"3.49.0"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1988,10]]},"abstract":"<jats:p>Since both glucocorticoids and catecholamines are involved in the regulation of normal blood pressure, we investigated the modulation of beta-adrenergic receptors of cultured rat arterial smooth muscle cells by glucocorticoids. The synthetic glucocorticoids dexamethasone and RU 28362, at 10(-8) M concentration, increased maximum beta-adrenergic binding but had no effect on the dissociation constant (Kd). Each steroid caused an increase in maximum [3H]dihydroalprenolol binding over the concentration range of 10(-8) to 10(-6) M, but not at 10(-9) M. The glucocorticoid effect on beta-adrenergic receptors of arterial smooth muscle cells required a minimum of 20 hours of incubation in the presence of the steroid and was significantly inhibited by cycloheximide (10 micrograms\/ml), indicating that the glucocorticoid effect required protein synthesis. The effect of dexamethasone on [3H]dihydroalprenolol binding was significantly inhibited by the glucocorticoid antagonist RU 38486. Basal and agonist-stimulated cyclic adenosine 3',5'-monophosphate (cAMP) levels in arterial smooth muscle cells, before and after glucocorticoid treatment, were measured as an indicator of the physiological significance of the observed glucocorticoid-induced increase in beta-adrenergic receptor binding. While causing no change in the basal cAMP level, treatment of arterial smooth muscle cells with 10(-6) M dexamethasone for 24 hours increased the 10(-6) M isoproterenol-stimulated cAMP levels.<\/jats:p>","DOI":"10.1161\/01.hyp.12.4.393","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:23:24Z","timestamp":1339460604000},"page":"393-398","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":30,"title":["Glucocorticoid modulation of beta-adrenergic receptors of cultured rat arterial smooth muscle cells."],"prefix":"10.1161","volume":"12","author":[{"given":"A","family":"Jazayeri","sequence":"first","affiliation":[{"name":"Department of Psychiatry and Behavioral Sciences, University of Texas Medical Branch, Galveston 77550."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"3rd","given":"W J","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Behavioral Sciences, University of Texas Medical Branch, Galveston 77550."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.HYP.12.4.393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:37:51Z","timestamp":1715535471000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.12.4.393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,10]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1988,10]]}},"alternative-id":["10.1161\/01.HYP.12.4.393"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.12.4.393","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,10]]},"assertion":[{"value":"1988-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}