{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T16:03:15Z","timestamp":1761580995027},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6_pt_2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1989,6]]},"abstract":"<jats:p>alpha-Adrenergic receptor subtypes were investigated using [3H]prazosin, an alpha 1 selective antagonist, and the alpha 2 selective antagonist [3H]rauwolscine in a smooth muscle plasma membrane enriched microsomal fraction prepared from rabbit intrarenal arterial vasculature. Both radioligands displayed single components on Scatchard analysis. The specific binding of [3H]prazosin was of high affinity (0.54 +\/- 0.04 nM) with a maximum binding capacity (Bmax) of 212 +\/- 15 fmol\/mg protein. The maximum number of [3H]rauwolscine binding sites was 64 +\/- 4 fmol\/mg of protein with a dissociation constant (Kd) of 5.60 +2- 0.27 nM. Binding of both radioligands was rapid, saturable, and specific. alpha 1- and alpha 2-adrenergic receptors in the intrarenal arterial membrane preparation were also characterized at 2-, 4-6-, and 10-12-week intervals during the course of development and maintenance of chronic two-kidney, one clip (2K1C) Goldblatt hypertension and in age-matched sham-operated normotensive control rabbits. The alpha 1-adrenergic receptor affinity for [3H]prazosin binding in hypertensive rabbits was significantly increased in the stenotic, but not contralateral, kidney at 2 weeks; however, at 6 weeks the receptor affinity of both kidneys was significantly increased compared with those of the normotensive control group. No difference in alpha 1-adrenergic receptor affinity was seen at 12 weeks, and there were no changes in Bmax at any of the weekly intervals. Neither the Kd, nor Bmax, for [3H]rauwolscine in either kidney showed a significant difference between hypertensive rabbits and normotensive control rabbits. These studies demonstrate the existence in the rabbit intrarenal arterial vasculature of binding sites with alpha 1- and alpha 2-adrenergic receptor specificity.(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1161\/01.hyp.13.6.851","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:23:24Z","timestamp":1339460604000},"page":"851-858","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":12,"title":["Characterization of intrarenal arterial adrenergic receptors in renovascular hypertension."],"prefix":"10.1161","volume":"13","author":[{"given":"N D","family":"McElroy","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B G","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Minnesota Medical School, Minneapolis 55455."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.HYP.13.6.851","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:48:28Z","timestamp":1715536108000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.13.6.851"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,6]]},"references-count":0,"journal-issue":{"issue":"6_pt_2","published-print":{"date-parts":[[1989,6]]}},"alternative-id":["10.1161\/01.HYP.13.6.851"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.13.6.851","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,6]]},"assertion":[{"value":"1989-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}