{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:18:50Z","timestamp":1771643930553,"version":"3.50.1"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6_pt_2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:p>Endothelins (ETs) are 21-amino acid peptides with two disulfide bonds that have powerful vasoactive properties. We have previously shown the presence of a specific, high-affinity, saturable receptor for porcine or human endothelin (ET-1) in cultured calf zona glomerulosa cells. ET-1 was a stimulator of aldosterone secretion although not as powerful as angiotensin II. Incubations of cultured calf zona glomerulosa cells with Sarafotoxin S6b (S6b), a snake venom that has a structure highly homologous to ET-1, stimulated aldosterone secretion with a potency similar to that of ET-1. Binding of [125I]ET-1 to the adrenal receptor gave a Kd of 0.17 +\/- 0.05 nM and a Bmax of 36 +\/- 8.5 fmol\/well (n = 4). Displacement of [125I]ET-1 by unlabeled ETs and S6b showed that the concentrations needed to displace 50% of the tracer were 0.3 nM for ET-1, 0.3 nM for ET-2, 10 nM for S6b, and 100 nM for ET-3. Binding of [125I]S6b to cultured adrenal cells revealed a receptor with a Kd of 0.05 +\/- 0.01 nM and a Bmax of 8 +\/- 2 fmol\/well (n = 4). Displacement of [125I]S6b by unlabeled ETs and S6b showed that the concentrations needed to displace 50% of the tracer were 0.03 nM for S6b, 0.06 nM for ET-1, 0.04 nM for ET-2, and 0.05 nM for ET-3. Unlabeled ET-1 and ET-2 preferentially down-regulated the binding of [125I]ET-1, and S6b preferentially down-regulated the binding of [125I]S6b.(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1161\/01.hyp.15.6.744","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:23:24Z","timestamp":1339460604000},"page":"744-747","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["Endothelin receptor subtypes and stimulation of aldosterone secretion."],"prefix":"10.1161","volume":"15","author":[{"given":"C E","family":"Gomez-Sanchez","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University of South Florida Health Sciences Center, Tampa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E N","family":"Cozza","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of South Florida Health Sciences Center, Tampa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M F","family":"Foecking","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of South Florida Health Sciences Center, Tampa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Chiou","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of South Florida Health Sciences Center, Tampa."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M W","family":"Ferris","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of South Florida Health Sciences Center, Tampa."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.HYP.15.6.744","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:36:56Z","timestamp":1715535416000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.15.6.744"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":0,"journal-issue":{"issue":"6_pt_2","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["10.1161\/01.HYP.15.6.744"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.15.6.744","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]},"assertion":[{"value":"1990-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}