{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:53:25Z","timestamp":1758819205756},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2_supplement","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1992,2]]},"abstract":"<jats:p>The vasoconstrictor effect, the binding, and the response of inositol phosphates to endothelin-1 (ET-1) were investigated in blood vessels of deoxycorticosterone acetate (DOCA)-salt hypertensive rats within 2 weeks of development of hypertension and in uninephrectomized control rats. In DOCA-salt and uninephrectomized rats, plasma levels of endothelin were similar (1.2 +\/- 0.1 fmol\/ml). Thoracic aorta and mesenteric artery rings devoid of endothelium presented significantly decreased responses to increasing concentrations of ET-1. Binding of ET-1 to mesenteric artery membranes was significantly lower in DOCA-salt rats (106 +\/- 22 fmol\/mg protein) than in uninephrectomized rats (172 +\/- 19 fmol\/mg protein, p less than 0.05), whereas affinity was similar. Phosphoinositide metabolism was examined in aorta and mesenteric arteries after incubation with [3H]myoinositol. Inositol phosphates were separated by high-performance liquid chromatography. In response to 100 nmol\/l ET-1, accumulation of inositol 1,4,5-trisphosphate after 20 seconds and of inositol monophosphate, inositol bisphosphate, and inositol 1,3,4-triphosphate after 30 minutes (in the presence of 25 mmol\/l LiCl) were significantly lower in DOCA-salt hypertensive than in uninephrectomized control rats, in both aorta and mesenteric arteries. In conclusion, decreased density of ET-1 receptors in DOCA-salt hypertensive rats results in decreased activation of phospholipase C and, consequently, reduced vasoconstriction induced by ET-1. Because the decrease in vasoconstrictor effects of ET-1 is found in the absence of endothelium, it is likely that receptor downregulation rather than prior receptor occupancy underlies these findings.<\/jats:p>","DOI":"10.1161\/01.hyp.19.2_suppl.ii98","type":"journal-article","created":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T23:35:38Z","timestamp":1343864138000},"update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":32,"title":["Endothelin vascular receptors and responses in deoxycorticosterone acetate-salt hypertensive rats."],"prefix":"10.1161","volume":"19","author":[{"given":"P V","family":"Nguyen","sequence":"first","affiliation":[{"name":"Experimental Hypertension Laboratory, Clinical Research Institute of Montreal, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Parent","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, Clinical Research Institute of Montreal, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L Y","family":"Deng","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, Clinical Research Institute of Montreal, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J P","family":"Fl\u00fcckiger","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, Clinical Research Institute of Montreal, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Thibault","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, Clinical Research Institute of Montreal, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E L","family":"Schiffrin","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, Clinical Research Institute of Montreal, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.HYP.19.2_Suppl.II98","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:50:00Z","timestamp":1715536200000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.19.2_Suppl.II98"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2]]},"references-count":0,"journal-issue":{"issue":"2_supplement","published-print":{"date-parts":[[1992,2]]}},"alternative-id":["10.1161\/01.HYP.19.2_Suppl.II98"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.19.2_suppl.ii98","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,2]]},"assertion":[{"value":"1992-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}