{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T18:15:53Z","timestamp":1715537753685},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6_pt_2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:p>In previous studies a decreased responsiveness to endothelin-1 (ET-1) of conduit arteries and resistance vessels of deoxycorticosterone acetate (DOCA)-salt hypertensive rats was found in comparison with uninephrectomized controls. Decreased isometric force, number of receptors, and inositol phosphate accumulation were reported in the DOCA-salt animals. In the present study effects of ET-1 on cytosolic free calcium, inositol phosphates, and 1,2-diacylglycerol were investigated in blood vessels of DOCA-salt hypertensive rats. Basal cytosolic free calcium, measured with the fluorescent dye fura-2, was 201 +\/- 41 nmol\/l in mesenteric arteries of DOCA-salt rats and 45 +\/- 9 nmol\/l in uninephrectomized controls (p less than 0.01). The maximal response of cytosolic free calcium (to 30 nmol\/l ET-1) was 176 +\/- 22% of the basal value for DOCA-salt and 242 +\/- 6% for uninephrectomized rats (p less than 0.05). The concentration giving 50% of the maximum response was 9.0 and 6.5 nmol\/l for DOCA-salt rats and controls, respectively. Inositol phosphate production after stimulation with 100 nmol\/l ET-1 in the presence of LiCl was lower by at least 30% (p less than 0.01) in both aorta and mesenteric arteries of DOCA-salt hypertensive versus control rats. Basal levels of diacylglycerol in aorta were similar in DOCA-salt rats and in controls and did not respond to a 100 nmol\/l ET-1 stimulation in the DOCA-salt rats, in contrast to the increase found in the control uninephrectomized rats (p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1161\/01.hyp.19.6.743","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"743-748","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":18,"title":["Calcium, phosphoinositide, and 1,2-diacylglycerol responses of blood vessels of deoxycorticosterone acetate-salt hypertensive rats to endothelin-1."],"prefix":"10.1161","volume":"19","author":[{"given":"J P","family":"Fl\u00fcckiger","sequence":"first","affiliation":[{"name":"Experimental Hypertension Laboratory, University of Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P V","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, University of Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Li","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, University of Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X P","family":"Yang","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, University of Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E L","family":"Schiffrin","sequence":"additional","affiliation":[{"name":"Experimental Hypertension Laboratory, University of Montreal, Quebec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.HYP.19.6.743","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:15:06Z","timestamp":1715534106000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.19.6.743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6]]},"references-count":0,"journal-issue":{"issue":"6_pt_2","published-print":{"date-parts":[[1992,6]]}},"alternative-id":["10.1161\/01.HYP.19.6.743"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.19.6.743","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6]]},"assertion":[{"value":"1992-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}