{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T06:11:51Z","timestamp":1778134311051,"version":"3.51.4"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:p>Chromogranins A and B are major soluble proteins in chromaffin granules. Their adrenomedullary content is increased in the spontaneously (genetic) hypertensive rat. Is augmented catecholamine vesicular storage of the chromogranins a specific feature of genetic hypertension? To explore this question, we measured chromogranin A immunoreactivity, using a novel, synthetic peptide radioimmunoassay, in rat adrenal medullas 4-6 weeks after induction of the two-kidney, one clip Goldblatt model of renovascular hypertension and in unmanipulated control animals. We also measured messenger RNAs of chromogranins A and B and dopamine beta-hydroxylase by Northern blot. Immunoreactive adrenal chromogranin A was 3.3-fold higher (p &lt; 0.01) in clipped rat adrenals. Adrenal catecholamine concentrations and phenylethanolamine-N-methyltransferase activity were also higher in clipped rats. Adrenal dopamine beta-hydroxylase activity (both membrane-bound and soluble forms) and corticosterone (glucocorticoid) concentration did not significantly differ between the groups. Adrenal medullary chromogranin A messenger RNA levels in clipped rats were 3.2-fold higher (p = 0.029) than those in the control group, and chromogranin B messenger RNA levels were 4.6-fold higher (p = 0.05). Dopamine beta-hydroxylase messenger RNA levels were 2.9-fold higher (p = 0.038). Thus, augmented synthesis and storage of adrenomedullary chromogranins A and B, catecholamines, and their biosynthetic enzymes appear to be characteristic of both acquired and genetic hypertension.<\/jats:p>","DOI":"10.1161\/01.hyp.21.5.674","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"674-679","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":33,"title":["Catecholamine secretory vesicles. Augmented chromogranins and amines in secondary hypertension."],"prefix":"10.1161","volume":"21","author":[{"given":"M A","family":"Takiyyuddin","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"De Nicola","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F B","family":"Gabbai","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T Q","family":"Dinh","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M G","family":"Ziegler","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E L","family":"Sabban","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R J","family":"Parmer","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D T","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California, San Diego 92161."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.HYP.21.5.674","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:23:54Z","timestamp":1715534634000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.21.5.674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1993,5]]}},"alternative-id":["10.1161\/01.HYP.21.5.674"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.21.5.674","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,5]]},"assertion":[{"value":"1993-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}