{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:06:56Z","timestamp":1715558816239},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6_pt_1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1994,6]]},"abstract":"<jats:p>This study examined the contribution of phosphatidylinositol metabolism and the efficacy of protein kinase C-mediated desensitization in the exaggerated alpha 1b-adrenergic receptor-mediated inositol phosphate response in the aorta of the deoxycorticosterone acetate (DOCA)-salt rat model of hypertension. The basal accumulation of inositol phosphates and the basal incorporation of [3H]myo-inositol in the phosphatidylinositol lipid pool were significantly higher in the aorta of these hypertensive rats. A positive correlation (r = .88, P &lt; .01) was demonstrated between basal inositol phosphate levels and the [3H]myo-inositol-labeled phosphatidylinositol lipid pool. In hypertensive rats, alpha 1b-adrenergic receptor-mediated inositol phosphate production in response to phenylephrine was significantly higher compared with normotensive rats. Despite the normalization of phenylephrine-mediated inositol phosphate production to the [3H]myo-inositol-labeled phosphatidylinositol lipid pool, the alpha 1b-adrenergic response remained significantly higher in the hypertensive rats. Phorbol ester activation of protein kinase C attenuated to a lesser extent phenylephrine-mediated inositol phosphate production (40%) in the aorta of hypertensive rats compared with the 80% attenuation observed in the aorta of normotensive rats. This desensitization was inhibited in both groups by the protein kinase C inhibitor staurosporine. The blunted desensitization of the alpha 1b-adrenergic receptor by protein kinase C activation was not associated with a decrease in protein kinase C activity in the hypertensive rats, because aortic strips from these animals were more responsive to phorbol ester activation than aortic strips from normotensive animals.(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1161\/01.hyp.23.6.722","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"722-728","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":7,"title":["Altered protein kinase C regulation of phosphoinositide-coupled receptors in deoxycorticosterone acetate-salt hypertensive rats."],"prefix":"10.1161","volume":"23","author":[{"given":"A","family":"Calderone","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Physiology, Universit\u00e9 de Montr\u00e9al, Qu\u00e9bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Oster","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Physiology, Universit\u00e9 de Montr\u00e9al, Qu\u00e9bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Moreau","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Physiology, Universit\u00e9 de Montr\u00e9al, Qu\u00e9bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J L","family":"Rouleau","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Physiology, Universit\u00e9 de Montr\u00e9al, Qu\u00e9bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D J","family":"Stewart","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Physiology, Universit\u00e9 de Montr\u00e9al, Qu\u00e9bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"de Champlain","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Physiology, Universit\u00e9 de Montr\u00e9al, Qu\u00e9bec, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.HYP.23.6.722","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:09:11Z","timestamp":1715533751000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.23.6.722"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6]]},"references-count":0,"journal-issue":{"issue":"6_pt_1","published-print":{"date-parts":[[1994,6]]}},"alternative-id":["10.1161\/01.HYP.23.6.722"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.23.6.722","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6]]},"assertion":[{"value":"1994-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}