{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:06:09Z","timestamp":1715558769894},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1994,9]]},"abstract":"<jats:p>We investigated sodium-proton (Na(+)-H+) exchange activity in transgenic TGR(mRen-2)27 rats, a strain showing fulminant hypertension after the mouse Ren-2d renin gene has been integrated into its genome, in age-matched normotensive Sprague-Dawley (SD) rats, in spontaneously hypertensive rats (SHR) from the M\u00fcnster strain, and in normotensive Wistar-Kyoto (WKY) rats. From each strain Na(+)-H+ exchange activity was determined in lymphocytes using the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) by measuring the recovery rate of cytosolic pH (pHi) after intracellular acidification. Resting pHi was not significantly different in transgenic rats (n = 10) compared with SD rats (n = 10) (7.305 +\/- 0.038 versus 7.337 +\/- 0.031; mean +\/- SEM), but resting pHi was significantly lower in lymphocytes from SHR (n = 12) compared with their normotensive WKY counterparts (n = 12) (7.232 +\/- 0.030 versus 7.377 +\/- 0.022; P &lt; .01). Na(+)-H+ exchange activity was significantly lower in lymphocytes from transgenic rats compared with SD rats (5.102 +\/- 0.561 versus 7.385 +\/- 0.491 x 10(-3) dpHi\/s; P &lt; .01), whereas Na(+)-H+ exchange was significantly enhanced in lymphocytes from SHR compared with WKY rats (5.564 +\/- 0.432 versus 3.921 +\/- 0.433 x 10(-3) dpHi\/s; P &lt; .05). The apparent half-maximal activation of Na(+)-H+ exchange was not significantly different in the strains tested. The present study indicates that hypertension in transgenic rats is not related to Na(+)-H+ exchange overactivity.<\/jats:p>","DOI":"10.1161\/01.hyp.24.3.357","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"357-361","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Reduced sodium-proton exchange activity in lymphocytes from transgenic rats."],"prefix":"10.1161","volume":"24","author":[{"given":"M","family":"Tepel","sequence":"first","affiliation":[{"name":"Medizinische Universit\u00e4ts-Poliklinik, University of M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Klaus","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts-Poliklinik, University of M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Laukemper","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts-Poliklinik, University of M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Zidek","sequence":"additional","affiliation":[{"name":"Medizinische Universit\u00e4ts-Poliklinik, University of M\u00fcnster, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/pdf\/10.1161\/01.HYP.24.3.357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T17:21:45Z","timestamp":1715534505000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.24.3.357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,9]]}},"alternative-id":["10.1161\/01.HYP.24.3.357"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.24.3.357","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,9]]},"assertion":[{"value":"1994-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}