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The PKC inhibitor staurosporine (10 to 100 nmol\/L) increased the expression of ecNOS mRNA, assessed by Northern analysis, in a dose-dependent manner. A newly developed, more specific PKC inhibitor, chelerythrine (1 to 3 \u03bcmol\/L), also increased the level of ecNOS mRNA. Incubation of BAEC with phorbol 12-myristate 13-acetate (100 nmol\/L) for 24 hours, which downregulates PKC, increased ecNOS mRNA expression. The protein content of ecNOS, assessed by Western analysis, was also increased in staurosporine-treated or chelerythrine-treated BAEC. The release of nitrogen oxides from staurosporine-treated or chelerythrine-treated cells both under basal conditions and in response to calcium ionophore A23187 was significantly increased (<jats:italic>P<\/jats:italic>&lt;.05). In conclusion, the present study suggests that regulation of ecNOS is mediated by PKC. The increased release of nitric oxide induced by PKC inhibition may play a protective role against atherogenic process.<\/jats:p>","DOI":"10.1161\/01.hyp.25.3.415","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"415-420","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":115,"title":["Regulation of Endothelial Constitutive Nitric Oxide Synthase by Protein Kinase C"],"prefix":"10.1161","volume":"25","author":[{"given":"Yuichi","family":"Ohara","sequence":"first","affiliation":[{"name":"From the Division of Cardiology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan S.","family":"Sayegh","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jay J.","family":"Yamin","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David G.","family":"Harrison","sequence":"additional","affiliation":[{"name":"From the Division of Cardiology, Emory University School of Medicine, and the Atlanta Veterans Administration Medical Center, Atlanta, Georgia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2910329"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90874-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3014651"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/334661a0"},{"key":"e_1_3_2_5_2","first-page":"1112","volume":"11","year":"1988","unstructured":"Bendhack LM, Sharma RV, Bhalla RC. 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