{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T13:45:22Z","timestamp":1774532722968,"version":"3.50.1"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1995,4]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            We hypothesized that the gene expression of angiotensinogen, angiotensin-converting enzyme, and angiotensin II type 1 receptor, in addition to renin, is increased in kidneys after renal artery stenosis. Two-kidney, one clip renovascular hypertension was initiated in Sprague-Dawley rats by clipping of the left renal artery; control rats were sham operated. Blood pressure was not changed for the first 2 days after clipping but was elevated on day 4 (mean arterial pressure, 104\u00b14 versus 87\u00b12 mm Hg in sham-operated control rats,\n            <jats:italic>P<\/jats:italic>\n            &lt;.002) and increased further during the next 24 days. Rats were killed 2, 4, 7, 14, and 28 days after clipping or sham operation, and poly(A)\n            <jats:sup>+<\/jats:sup>\n            -purified renal cortical RNA was analyzed by Northern blotting. Autoradiographs were quantitated by densitometry and normalized for the expression of a housekeeping gene. Renin expression was increased in the clipped kidney (by 149% on day 2) and decreased in the nonclipped kidney (by 82% on day 2), compared with kidneys of control rats. Expression of the angiotensin-converting enzyme was increased in clipped kidneys from the first day after clipping (158%) and throughout the experiment (66% on day 28), but was unchanged or slightly decreased in nonclipped kidneys. Angiotensinogen mRNA showed little change. Angiotensin II type 1 receptor expression was decreased in nonclipped kidneys but unchanged during the first 7 days in clipped kidneys. Our results show that components of the renin-angiotensin system other than renin are also differentially expressed in clipped kidneys. Increased expression of the angiotensin-converting enzyme in poststenotic kidneys occurs very early in the development of renovascular hypertension and may contribute to increased intrarenal angiotensin II formation.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.25.4.674","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"674-678","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":24,"title":["Expression of Angiotensin-Converting Enzyme in Renovascular Hypertensive Rat Kidney"],"prefix":"10.1161","volume":"25","author":[{"given":"Monika","family":"Mai","sequence":"first","affiliation":[{"name":"From the Nephrology Laboratory, Department of Medicine IV, University of Erlangen, and Max-Delbru\u0308ck Center, Berlin-Buch (J.W.), Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl F.","family":"Hilgers","sequence":"additional","affiliation":[{"name":"From the Nephrology Laboratory, Department of Medicine IV, University of Erlangen, and Max-Delbru\u0308ck Center, Berlin-Buch (J.W.), Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju\u0308rgen","family":"Wagner","sequence":"additional","affiliation":[{"name":"From the Nephrology Laboratory, Department of Medicine IV, University of Erlangen, and Max-Delbru\u0308ck Center, Berlin-Buch (J.W.), Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes F. E.","family":"Mann","sequence":"additional","affiliation":[{"name":"From the Nephrology Laboratory, Department of Medicine IV, University of Erlangen, and Max-Delbru\u0308ck Center, Berlin-Buch (J.W.), Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut","family":"Geiger","sequence":"additional","affiliation":[{"name":"From the Nephrology Laboratory, Department of Medicine IV, University of Erlangen, and Max-Delbru\u0308ck Center, Berlin-Buch (J.W.), Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","unstructured":"Robertson JIS. Renin and the pathophysiology of renovascular hypertension. In: Robertson JIS Nicholls MG eds. The Renin-Angiotensin System. London UK: Mosby; 1993:55.1-55.34."},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.24.2.157"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-198902000-00005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.22.5.754"},{"key":"e_1_3_2_5_2","first-page":"H1871","volume":"264","year":"1993","unstructured":"Veelken R, Hilgers KF, Leonard ME, Scrogin KE, Ruhe R, Mann JFE, Luft FC. A highly selective cardiorenal serotonergic 5-HT-3 mediated reflex in rats. 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