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We demonstrate that human vascular smooth muscle cells express abundant mRNA for vascular permeability\/endothelial growth factor. Vascular permeability factor is a 34- to 42-kD glycoprotein that markedly increases vascular endothelial permeability and is a potent endothelial mitogen. Ang II potently induced a concentration-dependent (maximal, 10<jats:sup>\u22127<\/jats:sup>mol\/L) and time-dependent increase in vascular permeability factor mRNA expression by human vascular smooth muscle cells that was maximal after 3 hours and diminished by 24 hours. Ang II\u2013induced vascular permeability factor mRNA expression by human vascular smooth muscle cells was inhibited by the specific Ang II receptor antagonist losartan (DuP 753), confirming that this is an Ang II receptor subtype 1\u2013mediated event. These results describe a new action of Ang II on human vascular smooth muscle, notably the induction of vascular permeability factor mRNA expression. The wide spectrum and potent activity of vascular permeability factor suggest a novel mechanism whereby Ang II could locally and directly influence the permeability, growth, and function of the vascular endothelium independent of changes in hemodynamics.<\/jats:p>","DOI":"10.1161\/01.hyp.25.5.913","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"913-917","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":220,"title":["Angiotensin II Increases Vascular Permeability Factor Gene Expression by Human Vascular Smooth Muscle Cells"],"prefix":"10.1161","volume":"25","author":[{"given":"Bryan","family":"Williams","sequence":"first","affiliation":[{"name":"From the Department of Medicine, University of Leicester (UK) School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Quinn","family":"Baker","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, University of Leicester (UK) School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Gallacher","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, University of Leicester (UK) School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lodwick","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, University of Leicester (UK) School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/362801a0"},{"key":"e_1_3_2_2_2","first-page":"111","volume":"52","year":"1977","unstructured":"Beilin LJ, Goldby FS. 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