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Increasing Na\/Cl concentrations from 86\/67 to 106\/87 mmol\/L had no further effect on renin secretion. [Cl]<jats:sub>1\/2<\/jats:sub>, the chloride concentration producing the half-maximal effect, was 30 mmol\/L. Addition of 50 \u03bcmol\/L furosemide to the luminal fluid caused renin secretion to become essentially independent of macula densa NaCl concentration. This effect was due to both an increase of renin secretion at high NaCl concentrations and a decrease of renin release at low NaCl concentrations. Verapamil added to the superfusate at a concentration of 1 \u03bcmol\/L also abolished NaCl dependency of renin secretion; most of this effect was due to an increase of renin release at high luminal NaCl. These results suggest that Na-2Cl-K cotransport and calcium flux through voltage-gated channels are two mechanisms required for the expression of NaCl-dependent renin release. Identification of the cellular localizations of these two critical membrane proteins in the renin control pathway requires further study.<\/jats:p>","DOI":"10.1161\/01.hyp.26.1.137","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"137-142","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":72,"title":["Effects of Furosemide and Verapamil on the NaCl Dependency of Macula Densa\u2013Mediated Renin Secretion"],"prefix":"10.1161","volume":"26","author":[{"given":"Xiao-Rui","family":"He","sequence":"first","affiliation":[{"name":"From the Departments of Physiology and Internal Medicine, the University of Michigan, Ann Arbor."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne G.","family":"Greenberg","sequence":"additional","affiliation":[{"name":"From the Departments of Physiology and Internal Medicine, the University of Michigan, Ann Arbor."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josie P.","family":"Briggs","sequence":"additional","affiliation":[{"name":"From the Departments of Physiology and Internal Medicine, the University of Michigan, Ann Arbor."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju\u0308rgen","family":"Schnermann","sequence":"additional","affiliation":[{"name":"From the Departments of Physiology and Internal Medicine, the University of Michigan, Ann Arbor."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3306925"},{"key":"e_1_3_2_2_2","first-page":"F186","volume":"259","year":"1990","unstructured":"Lorenz JN, Weihprecht H, Schnermann J, Sk\u00f8tt O, Briggs JP. 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