{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:20:21Z","timestamp":1791069621541,"version":"4.1.0"},"reference-count":12,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:p><jats:italic>Abstract<\/jats:italic>Vascular responsiveness to 5-hydroxytryptamine (5-HT) is dramatically increased in hypertension. The hypothesis that augmented vasoconstriction to 5-HT in hypertension is due to a change in receptor subtype on vascular myocytes was tested. Mesenteric arteries from deoxycorticosterone acetate (DOCA)-salt hypertensive (systolic blood pressure &gt;180 mm Hg) and sham normotensive (systolic blood pressure &lt;130 mm Hg) rats were mounted in isolated tissue baths for measurement of isometric contractile force. The receptor mediating contraction in isolated mesenteric arteries from sham and DOCA-salt hypertensive rats is a member of the 5-HT<jats:sub>2<\/jats:sub>family based on rank order of agonist potency (5-HT=\u03b1-methyl-5-HT [5-HT<jats:sub>2<\/jats:sub>receptor agonist]&gt;tryptamine&gt;5-hydroxykynuramine). 5-HT was approximately 10-fold more potent in contracting mesenteric arteries from DOCA-salt hypertensive rats compared with arteries from sham normotensive rats. The tryptophan metabolite kynuramine, which possesses significant contractile activity at the 5-HT<jats:sub>2B<\/jats:sub>receptor, contracted hypertensive arteries significantly (50% of 5-HT maximum) but not sham arteries. Ketanserin (5-HT<jats:sub>2A<\/jats:sub>antagonist) competitively inhibited contraction to 5-HT in arteries from normotensive rats (\u2212log dissociation constant [mol\/L]; p<jats:italic>K<\/jats:italic><jats:sub>B<\/jats:sub>=8.54) but not from hypertensive rats (p<jats:italic>K<\/jats:italic><jats:sub>B<\/jats:sub>&gt;6.5). Moreover, contraction to kynuramine was not blocked by ketanserin. Thus, under normal conditions, 5-HT<jats:sub>2A<\/jats:sub>receptors mediate contraction to 5-HT. However, in DOCA-salt hypertension, ketanserin-insensitive 5-HT<jats:sub>2<\/jats:sub>receptors, possibly 5-HT<jats:sub>2B<\/jats:sub>receptors, mediate mesenteric arterial contraction to 5-HT.<\/jats:p>","DOI":"10.1161\/01.hyp.26.6.1056","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:24:03Z","timestamp":1339460643000},"page":"1056-1059","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":32,"title":["5-Hydroxytryptamine<sub>2B<\/sub>Receptor Mediates Contraction in the Mesenteric Artery of Mineralocorticoid Hypertensive Rats"],"prefix":"10.1161","volume":"26","author":[{"given":"Stephanie W.","family":"Watts","sequence":"first","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Michigan State University, E Lansing (S.W.W.), and the Department of Physiology, The University of Michigan, Ann Arbor (L.G., R.C.W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Gilbert","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Michigan State University, E Lansing (S.W.W.), and the Department of Physiology, The University of Michigan, Ann Arbor (L.G., R.C.W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Clinton","family":"Webb","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Michigan State University, E Lansing (S.W.W.), and the Department of Physiology, The University of Michigan, Ann Arbor (L.G., R.C.W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1970.219.3.687"},{"key":"e_1_3_2_2_2","unstructured":"Turla MB Webb RC. Vascular responsiveness to 5-hydroxytryptamine in experimental hypertension. In: Fozard JR ed. The Peripheral Actions of 5-Hydroxytryptamine. Oxford UK: Oxford University Press; 1989:327-353."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.11.2.111"},{"key":"e_1_3_2_4_2","first-page":"465","volume":"241","year":"1987","unstructured":"Pomfret DW, Schenck KW, Fludzinski P, Cohen ML. Interaction of 5-hydroxykynurenamine, L-kynurenine and kynuramine with multiple serotonin receptors in smooth muscle. J Pharmacol Exp Ther. 1987;241:465-471.","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/S0026-895X(25)13628-6","volume":"43","year":"1993","unstructured":"Wainscott D, Cohen ML, Schenck KW, Audia JE, Nissen JS, Baez M, Kursar JD, Lucaites VL, Nelson DL. Pharmacological characteristics of the newly cloned rat 5-hydroxytryptamine2F receptor. Mol Pharmacol. 1993;43:419-426.","journal-title":"Mol Pharmacol"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/S0022-3565(25)38745-8","volume":"269","year":"1994","unstructured":"Watts SW, Webb RC. Mechanism of ergonovine-induced contraction in the mesenteric artery from deoxycorticosterone acetate-salt hypertensive rat. J Pharmacol Exp Ther. 1994;269:617-625.","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_3_2_7_2","unstructured":"Hoyer D. 5-Hydroxytryptamine receptors and effector coupling mechanism in peripheral tissues. In: Fozard JR ed. The Peripheral Actions of 5-Hydroxytryptamine. Oxford UK: Oxford University Press; 1989:72-99."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1957.tb00146.x"},{"key":"e_1_3_2_9_2","unstructured":"Johnson TD Clarke DE. In: Stone TW ed. Quinolinic Acid and the Kynuramines. Boca Raton Fla: CRC Press; 1989:213-226."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.75.6.7955146"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.6.6.887"},{"key":"e_1_3_2_12_2","unstructured":"Baxter GS Murphy OE Ellis E Tilford N Jones GJ Forbes I. Identification of a potent and selective antagonist at rat 5-HT2B receptors. In: Proceedings from the Third IUPHAR Satellite Meeting on Serotonin. 1994:75. Abstract A40."}],"container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.HYP.26.6.1056","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T19:22:26Z","timestamp":1743362546000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.26.6.1056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12]]},"references-count":12,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1995,12]]}},"alternative-id":["10.1161\/01.HYP.26.6.1056"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.26.6.1056","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"value":"0194-911X","type":"print"},{"value":"1524-4563","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12]]},"assertion":[{"value":"1995-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1995-09-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"1995-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}