{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T21:02:55Z","timestamp":1777582975591,"version":"3.51.4"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            Nitric oxide has a diuretic effect in vivo. We have shown that nitric oxide inhibits antidiuretic hormone\u2013stimulated osmotic water permeability in the collecting duct; however, the mechanism by which this occurs is unknown. We hypothesized that inhibition of antidiuretic hormone\u2013stimulated water permeability by nitric oxide in the collecting duct is the result of activation of cGMP-dependent protein kinase, which in turn decreases intracellular cAMP. To test this hypothesis, we microperfused cortical collecting ducts. Antidiuretic hormone\u2013stimulated water permeability was 317\u00b147 \u03bcm\/s (\n            <jats:italic>P<\/jats:italic>\n            &lt;.001). Addition of spermine NONOate, a nitric oxide donor, to the bath decreased water permeability to 74\u00b138 \u03bcm\/s (\n            <jats:italic>P<\/jats:italic>\n            &lt;.002). In the presence of LY 83583, an inhibitor of soluble guanylate cyclase, spermine NONOate did not change water permeability. Addition of spermine NONOate increased cGMP production (\n            <jats:italic>P<\/jats:italic>\n            &lt;.01). In the presence of the cGMP-dependent protein kinase inhibitor, spermine NONOate did not change water permeability. Since antidiuretic hormone increases water permeability by increasing cAMP, we hypothesized that nitric oxide inhibits water permeability by decreasing cAMP. In tubules pretreated with antidiuretic hormone, intracellular cAMP was 18.9\u00b13.9 fmol\/mm. In tubules treated with antidiuretic hormone and spermine NONOate, cAMP was 9.3\u00b11.7 fmol\/mm (\n            <jats:italic>P<\/jats:italic>\n            &lt;.03). We also examined the effect of spermine NONOate on dibutyryl-cAMP\u2013stimulated water permeability. In the presence of dibutyryl-cAMP, water permeability was 388\u00b130 \u03bcm\/s. Addition of spermine NONOate had no significant effect on water permeability. Time controls and inhibitors by themselves did not change antidiuretic hormone\u2013stimulated water permeability. We concluded that nitric oxide decreases antidiuretic hormone\u2013stimulated water permeability by increasing cGMP via soluble guanylate cyclase, activating cGMP-dependent protein kinase and decreasing cAMP.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.27.3.679","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:53:46Z","timestamp":1339462426000},"page":"679-683","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":67,"title":["Mechanism of the Nitric Oxide-Induced Blockade of Collecting Duct Water Permeability"],"prefix":"10.1161","volume":"27","author":[{"given":"Ne\u0301stor H.","family":"Garci\u0301a","sequence":"first","affiliation":[{"name":"From the Division of Hypertension and Vascular Research, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara A.","family":"Stoos","sequence":"additional","affiliation":[{"name":"From the Division of Hypertension and Vascular Research, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar A.","family":"Carretero","sequence":"additional","affiliation":[{"name":"From the Division of Hypertension and Vascular Research, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey L.","family":"Garvin","sequence":"additional","affiliation":[{"name":"From the Division of Hypertension and Vascular Research, Henry Ford Hospital, Detroit, Mich."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1992.tb00514.x"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajh\/4.3.260"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.22.4.535"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/hyp.25.4.860"},{"key":"e_1_3_2_5_2","first-page":"F344","volume":"264","year":"1993","unstructured":"Lahera V, Navarro J, Biondi ML, Ruilope LM, Romero JC. 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