{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:40:18Z","timestamp":1762004418116,"version":"3.40.3"},"reference-count":51,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1996,11]]},"abstract":"<jats:p>Previous studies have shown that the diuretic hormone atrial natriuretic peptide (ANP) also regulates the steroidogenic responsiveness in isolated Leydig cells from mouse and rat testes. In the present study, we examined the distribution of specific receptors for ANP and C-type natriuretic peptide (CNP) in the testicular compartments of 12-week-old Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). We used an in vitro autoradiographic procedure on slide-mounted frozen testicular sections to localize the receptors of the natriuretic peptide hormone family using<jats:sup>125<\/jats:sup>I-ANP and<jats:sup>125<\/jats:sup>I-CNP as radioligands. A high level of specific<jats:sup>125<\/jats:sup>I-ANP binding sites was localized largely in the Leydig cells of the interstitial compartment; other testicular cells were not significantly labeled. On the other hand, no significant difference was observed in<jats:sup>125<\/jats:sup>I-CNP binding sites in the testicular cells of SHR and WKY. Semiquantitative analysis of the binding sites indicated that the density of<jats:sup>125<\/jats:sup>I-ANP receptor binding in Leydig cells of WKY testis was ninefold higher than in those of SHR testis. A moderate level of<jats:sup>125<\/jats:sup>I-ANP binding was also observed in seminiferous tubules, particularly in the spermatids of both SHR and WKY.<jats:sup>125<\/jats:sup>I-ANP binding in WKY spermatids was approximately 2.5-fold higher than in SHR spermatids. Northern blot analysis showed that mRNA specific for guanylyl cyclase type A (Npra) was expressed at approximately twofold higher levels in WKY than in SHR testis. ANP (1\u00d710<jats:sup>\u22128<\/jats:sup>mol\/L) stimulated fourfold to fivefold increased levels of testosterone production in isolated Leydig cells from normotensive WKY compared with those from SHR. These findings support a new physiological role of ANP in Leydig cells, in which a functional relationship seems to exist between testicular ANP receptor expression and testosterone production and the state of hypertension in SHR.<\/jats:p>","DOI":"10.1161\/01.hyp.28.5.847","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:53:46Z","timestamp":1339462426000},"page":"847-853","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Differential Expression and Autoradiographic Localization of Atrial Natriuretic Peptide Receptor in Spontaneously Hypertensive and Normotensive Rat Testes: Diminution of Testosterone in Hypertension"],"prefix":"10.1161","volume":"28","author":[{"given":"Aditi A.","family":"Kapasi","sequence":"first","affiliation":[{"name":"the Departments of Biochemistry and Molecular Biology and of Pharmacology and Toxicology (J.R.P.), Medical College of Georgia School of Medicine, Augusta."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ravindra","family":"Kumar","sequence":"additional","affiliation":[{"name":"the Departments of Biochemistry and Molecular Biology and of Pharmacology and Toxicology (J.R.P.), Medical College of Georgia School of Medicine, Augusta."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James R.","family":"Pauly","sequence":"additional","affiliation":[{"name":"the Departments of Biochemistry and Molecular Biology and of Pharmacology and Toxicology (J.R.P.), Medical College of Georgia School of Medicine, Augusta."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailash N.","family":"Pandey","sequence":"additional","affiliation":[{"name":"the Departments of Biochemistry and Molecular Biology and of Pharmacology and Toxicology (J.R.P.), Medical College of Georgia School of Medicine, Augusta."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)94025-1"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1990.70.3.665"},{"key":"e_1_3_2_3_2","first-page":"219","volume":"60","year":"1991","unstructured":"Rosenzweig A, Seidman CE. Atrial natriuretic factor and related peptide hormones. Annu Rev Biochem. 1991;60:219-225.","journal-title":"Annu Rev Biochem"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-15-2-135"},{"key":"e_1_3_2_5_2","unstructured":"Espiner EA. Hormones of the cardiovascular system. In: De Groot J ed. Endocrinology. Philadelphia Pa: WB Saunders; 1995;3:2895-2916."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(81)90370-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2932797"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.69.2.1830518"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)92401-K"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(92)90065-B"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90259-F"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03518.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/341068a0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/338078a0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90513-8"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38352-8"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1672777"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.130.1.1309330"},{"key":"e_1_3_2_19_2","first-page":"675","volume":"238","year":"1978","unstructured":"Maack T, Suzuki M, Almeida FA, Nussenzveig D, Scarborough RM, McEnroe GA, Lewicki JA. Physiological role of silent atrial natriuretic factor receptor. Science. 1978;238:675-678.","journal-title":"Science"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)76554-5"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)80123-2"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(89)80158-5"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114893"},{"key":"e_1_3_2_24_2","first-page":"R453","volume":"261","year":"1991","unstructured":"Levin E, Frank H. Natriuretic peptides inhibit rat astroglial proliferation: mediation by C receptor. Am J Physiol. 1991;261:R453-R457.","journal-title":"Am J Physiol"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.263.5.C1001"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/hyp.25.2.227"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.133.5.8404664"},{"key":"e_1_3_2_28_2","first-page":"495","volume":"124","year":"1962","unstructured":"Hunter WM, Greenwood FC. Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature. 1962;124:495-496.","journal-title":"Nature"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00374a022"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53621-8"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1179\/014788889794651870"},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/S0022-3565(25)20464-5","volume":"258","year":"1991","unstructured":"Pauly JR, Marks MJ, Gross SD, Collins AC. An autoradiographic analysis of cholinergic receptors in mouse brain after chronic nicotine treatment. J Pharmacol Exp Ther. 1991;258:1127-1136.","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00591a005"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-109-4-1061"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1988.tb01063.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)37719-6"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90975-1"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)90295-0"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(86)80659-7"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.132.2.8425488"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)81312-9"},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1002\/j.1939-4640.1990.tb00179.x","volume":"11","year":"1990","unstructured":"Vollmar AM, Friedrich A, Schulz R. Atrial natriuretic peptide precursor material in rat testes. J Androl. 1990;11:471-475.","journal-title":"J Androl"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.3109\/01485019208987683"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2510301"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod44.2.225"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)95182-J"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.1.C106"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1530\/acta.0.1010154"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1536\/ihj.25.839"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-198909000-00005"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0197-0186(85)90064-6"}],"container-title":["Hypertension"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/01.HYP.28.5.847","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T21:36:56Z","timestamp":1743370616000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/01.HYP.28.5.847"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,11]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1996,11]]}},"alternative-id":["10.1161\/01.HYP.28.5.847"],"URL":"https:\/\/doi.org\/10.1161\/01.hyp.28.5.847","relation":{},"ISSN":["0194-911X","1524-4563"],"issn-type":[{"type":"print","value":"0194-911X"},{"type":"electronic","value":"1524-4563"}],"subject":[],"published":{"date-parts":[[1996,11]]},"assertion":[{"value":"1995-10-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1996-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}