{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T11:07:26Z","timestamp":1778843246899,"version":"3.51.4"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            Potassium efflux through Ca\n            <jats:sup>2+<\/jats:sup>\n            -sensitive K\n            <jats:sup>+<\/jats:sup>\n            channels (K\n            <jats:sub>Ca<\/jats:sub>\n            channels) is increased in arterial smooth muscle cells from hypertensive rats, but the molecular mechanism is unknown. The goal of this study was to compare the levels of K\n            <jats:sub>Ca<\/jats:sub>\n            channel current between aortic smooth muscle cells from adult Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) and then use Western blot methods and ribonuclease protection assays to examine the expression and mRNA levels for the K\n            <jats:sub>Ca<\/jats:sub>\n            channel in these same vascular tissues. Whole-cell patch-clamp methods indicated a larger component of K\n            <jats:sub>Ca<\/jats:sub>\n            channel current, sensitive to block by iberiotoxin (100 nmol\/L), in single aortic smooth muscle cells from SHR compared with WKY. Subsequent Western blot analysis using a site-specific antibody (anti\u2013\u03b1\n            <jats:sub>913\u2013926<\/jats:sub>\n            ) directed against the S9\/S10 linker of the \u03b1-subunit of the K\n            <jats:sub>Ca<\/jats:sub>\n            channel revealed a 125-kD immunoreactive band in lanes loaded with either WKY or SHR aortic muscle membranes. The immunoreactive density of this band, which corresponded to the known molecular size of the \u03b1-subunit, was 2.2-fold greater in lanes loaded with aortic smooth muscle membranes from the hypertensive animals. However, despite this evidence for an increased expression and functional enhancement of K\n            <jats:sub>Ca<\/jats:sub>\n            channels in aortic smooth muscle membranes of SHR, ribonuclease protection assays with a\n            <jats:sup>32<\/jats:sup>\n            P-labeled riboprobe targeted against the S9\/S10 linker of the K\n            <jats:sub>Ca<\/jats:sub>\n            channel \u03b1-subunit revealed no difference in mRNA levels for the \u03b1-subunit between WKY and SHR aortic tissue. These findings provide initial evidence that (1) an increased expression of K\n            <jats:sub>Ca<\/jats:sub>\n            channels may be a mechanism for the enhanced K\n            <jats:sub>Ca<\/jats:sub>\n            current in aortic smooth muscle membranes of SHR, and (2) the upregulation of K\n            <jats:sub>Ca<\/jats:sub>\n            channels in arterial muscle membranes during hypertension, which is regarded as a homeostatic mechanism for buffering vascular excitability, may rely on post-transcriptional events.\n          <\/jats:p>","DOI":"10.1161\/01.hyp.30.6.1403","type":"journal-article","created":{"date-parts":[[2012,6,12]],"date-time":"2012-06-12T00:53:46Z","timestamp":1339462426000},"page":"1403-1409","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":100,"title":["Increased Expression of Ca\n            <sup>2+<\/sup>\n            -Sensitive K\n            <sup>+<\/sup>\n            Channels in Aorta of Hypertensive Rats"],"prefix":"10.1161","volume":"30","author":[{"given":"Yanping","family":"Liu","sequence":"first","affiliation":[{"name":"From the Department of Physiology (Y.L., K.P., N.J.R.), Medical College of Wisconsin (Milwaukee), and Institute for Biochemical Pharmacology (H.-G.K.), Innsbruck, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kay","family":"Pleyte","sequence":"additional","affiliation":[{"name":"From the Department of Physiology (Y.L., K.P., N.J.R.), Medical College of Wisconsin (Milwaukee), and Institute for Biochemical Pharmacology (H.-G.K.), Innsbruck, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Guenther","family":"Knaus","sequence":"additional","affiliation":[{"name":"From the Department of Physiology (Y.L., K.P., N.J.R.), Medical College of Wisconsin (Milwaukee), and Institute for Biochemical Pharmacology (H.-G.K.), Innsbruck, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy J.","family":"Rusch","sequence":"additional","affiliation":[{"name":"From the Department of Physiology (Y.L., K.P., N.J.R.), Medical College of Wisconsin (Milwaukee), and Institute for Biochemical Pharmacology (H.-G.K.), Innsbruck, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.19.4.301"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.23.6.941"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1993.264.5.H1337"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.23.6.1091"},{"key":"e_1_3_2_5_2","first-page":"H1246","volume":"269","year":"1995","unstructured":"Liu Y, Jones AW, Sturek M. 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